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CBh-dCas9-Mll3SET

Plasmid

#108452

Purpose

Expression vector of CRISPR/dCas9 fused with SET domain of mouse MLL3 gene, driven by CBh promoter

Depositor

Bing Ren

Article

Yan et al Cell Res. 2018 Jan 9. pii: cr20181. doi:

Insert

dCas9, MLL3 (Kmt2c Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CBh

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-PtenC124S-Puro

Plasmid

#135677

Purpose

Constitutive expression (cMV promoter) of C124S mutant Pten cDNA, Puromycin selection

Depositor

Florian Karreth

Insert

Pten (Pten Mouse)

Use

Lentiviral

Tags

Expression

Mutation

C124S

Promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-IRES-hrGFP-mWnt3a

Plasmid

#89771

Purpose

Expresses mouse Wnt3a in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Rodriguez et al J Neurosci. 2014 Jul 30;34(31):10285-97.

Insert

mouse Wnt3a (Wnt3a Mouse)

Use

AAV

Tags

None

Expression

Mutation

Promoter

CMV

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pHR SFFVp BFP-Erk D319N

Plasmid

#102350

Purpose

Lentiviral expressing translational fusion between blue fluorescent protein and Erk2 bearing the D319N mutation

Depositor

Jared Toettcher

Article

Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

Insert

Erk2 (Mapk1 Mouse)

Use

Lentiviral

Tags

BFP

Expression

Mutation

Aspartic acid 319 was mutated to asparagine

Promoter

SFFV

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-MYO10-HMM

Plasmid

#87256

Purpose

Expression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO10 (Myo10 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBSFI-myr-akt-T308D/S473D

Plasmid

#49192

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Expression

Mutation

T308D; S473D

Promoter

T7

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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Cas9-GFP_sg_mAMPKa2

Plasmid

#79005

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Prkaa2 (Prkaa2 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pCAGGS-Stargazin-mEGFP-iLID

Plasmid

#178523

Purpose

Expresses Stargazin-mEGFP-iLID in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Stargazin-mEGFP-iLID

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Bcl11b

Plasmid

#139831

Purpose

doxycycline-inducible expression of mouse Bcl11b in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Bcl11b (Bcl11b Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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p178 Grg-1L Myc His

Plasmid

#11067

Depositor

Ramesh Shivdasani

Article

Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

Insert

Grg1-L (Tle1 Mouse)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-mSOX9-2

Plasmid

#40645

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSOX9-2 (Sox9 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-Smad4

Plasmid

#89829

Purpose

Knockdown human Smad4 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

Smad4 shRNA (SMAD4 Human, Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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mFGF4

Plasmid

#22085

Depositor

Gail Martin

Article

Hebert et al Dev Biol. 1990 Apr . 138(2):454-63.

Insert

FGF4 (Fgf4 Mouse)

Use

Bacterial

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
tetO-Bcl2-IRES-tdtomato(TET005)

Plasmid

#117857

Purpose

This plasmid expresses Bcl2 under the activation of tTA trans-activator along with fluorescent marker tdTomato.

Depositor

C. Ron Yu

Article

Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

Insert

Bcl2 (Bcl2 Mouse)

Use

Tta/teto

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-3a

Plasmid

#17994

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3a (Wnt3a Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
FUW-ubiquitin-ephrinB1-SV40-RFP (b1R)

Plasmid

#65446

Purpose

Expression of ephrinB1 and RFP in mammalian cells

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

ephrin B1 (Efnb1 Mouse)

Use

Lentiviral

Tags

SV40-RFP

Expression

Mammalian

Mutation

Promoter

UBQ

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
Setd3-Flag-pcDNA3.0

Plasmid

#49144

Purpose

mammalian expression of Flag tagged Setd3

Depositor

Jing H Wang

Article

Chen et al Haematologica. 2013 May;98(5):739-43. do

Insert

Setd3 (Setd3 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only
pBJ5-CnA

Plasmid

#11785

Depositor

Anjana Rao

Article

Clipstone et al J Biol Chem. 1994 Oct 21. 269(42):26431-

Insert

CnA (Ppp3ca Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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Bassoon scFv [L124/59]

Plasmid

#182078

Purpose

Mammalian Expression of Bassoon scFv. Derived from hybridoma L124/59.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Bassoon (Rattus norvegicus) (Bsn Mouse)

Use

Tags

Sortase, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag mHMGB2

Plasmid

#31610

Depositor

Yasuhiko Kawakami

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

Insert

HMGB2 (Hmgb2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only