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Plasmid#175447PurposeHiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTagsBiP Secretion Peptide and Thrombin-Protein A-10Hi…ExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only
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pASK-mSAN1-Streptag
Plasmid#117165PurposeExpresses Strep-tagged murine SAN1 nuclease in bacteriaDepositorInsertmSAN1 (Fam120b Mouse)
UseTagsStreptagExpressionBacterialMutationPromotertetR/tetAAvailable sinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
NET37 pEGFP-N2 (607)
Plasmid#61990Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET37 (KIAA1161 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-HS-NLRC4Δcard
Plasmid#177276PurposePurification of mouse NLRC4 (90-1024) from insect cellsDepositorInsertNLRC4 (Nlrc4 Mouse)
UseTagsHIS-SUMO-PreScissionExpressionInsectMutationI586T, N761S, M885V, K915R and F972L- please see …PromoterpolyhedrinAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_Crz1(19A)_CLASP_RGS2membrane
Plasmid#133085PurposePlasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.DepositorInsertCrz1*-CLASP
UseTagsExpressionBacterial and YeastMutationPromoterpADH1Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
NET97 pmRFP-N2 (641)
Plasmid#61994Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertAADACL1 (NCEH1 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
NET37 pmRFP-N2 (450)
Plasmid#61980Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET37 (KIAA1161 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-GpA
Plasmid#191238PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
UseTagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to CysteinePromoterT7 promoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
NET50 pmRFP-N2 (489)
Plasmid#61983Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET50 (DHRS7 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-NES-tdTomato-LINuS-WPRE
Plasmid#159894PurposeAAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signalDepositorInsertNES-tdTomato-LINuS
UseAAVTagsExpressionMammalianMutationPromoterEF1α promoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTE3987
Plasmid#137716PurposeExpresses dLbCas12a-RVRR-BE in mammalian cells.DepositorInsertdLbCas12a-RVRR-BE
UseTags6xHis, APOBEC-1, SV40 NLS, and UGIExpressionMammalianMutationG532A, K538V, Y542R, K595RPromoterCMVAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKsub(TA)DD-YPet-NES
Plasmid#84634PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertJNKsub(TA)DD-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
TAPBPL pmRFP-N2 (721)
Plasmid#62000Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertTAPBPL (TAPBPL Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
STT3A pmRFP (663)
Plasmid#61997Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertSTT3A (STT3A Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-UAP56 MUT
Plasmid#110124PurposeExpresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activityDepositorInsertUAP56 (DDX39B Human)
UseTagsFlagExpressionInsectMutation4 mutations (KSLN motif changed to RSFS)PromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-4T3-ZO-2-1st-PDZ-delta-NLS
Plasmid#27425DepositorInsertZonula Occludens 2 (TJP2 Human)
UseTagsGSTExpressionBacterialMutationDeletion of NLS-Nuclear Localization Signal: Nucl…PromoterAvailable sinceMay 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Notch1 LNG (CC→SS)-6MT
Plasmid#41739DepositorInsertNotch-1 Lin-Notch-glp (LNG) repeat-containing construct, CC->SS (Notch1 Mouse)
UseTags6xMycExpressionMammalianMutationContains murine Notch-1 leader peptide (aa 1-23) …PromoterAvailable sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNA 3C myc scfvHA
Plasmid#234991PurposeExpression of transmembrane region of Neuraminidase protein fused to an anti HA scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteinsDepositorInsertNA-3C-myc-scfvHA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Chim-Pchi-Q61A
Plasmid#231520PurposeExpresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generationDepositorInsertPchi HMG Q61A flanked with N- and C-termini of mSox2
UseLentiviralTagsExpressionMutationPoint mutation at position 61 Q to A of the HMG d…PromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only