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pcDNA3.1-cyto-KB1691-Nluc

Plasmid

#204326

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

cytosolic KB1691-Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRK7-FLAG-TSC1

Plasmid

#8995

Depositor

John Blenis

Article

Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

TSC1 (TSC1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pHT-ads

Plasmid

#47382

Purpose

Expresses ads under Pgrac promoter (inducible by IPTG) in bacteria

Depositor

Heng-Phon Too

Article

Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

Insert

ads

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Pgrac

Available since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pGR

Plasmid

#46002

Purpose

Reporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinose

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pMPRA1

Plasmid

#49349

Purpose

Basic backbone for transient transfection of Massively Parallel Reporter Assay (MPRA) libraries

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-5FLAG-natMX6

Plasmid

#19343

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

natMX6

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 25, 2008

Availability

Academic Institutions and Nonprofits only

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vYM140_PB-pEFa1-iaaM(P.s)-Hygro

Plasmid

#160043

Purpose

Expressing iaaM for converting L-Trp to IAM

Depositor

Michael Elowitz

Article

Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

Insert

iaaM (iaaM )

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-D1ER

Plasmid

#36325

Depositor

Roger Tsien, Amy Palmer

Article

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Insert

D1ER second generation cameleon (calcium sensor) targeted to ER

Use

Tags

KDEL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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HIV-1 Gag-mCherry

Plasmid

#85390

Purpose

Visualization marker for viral core to measure cellular uptake

Depositor

Gregory Melikyan

Article

Miyauchi et al Biochem J. 2011 Mar 15;434(3):559-69. do

Insert

HIV-1 gag

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-FHC

Plasmid

#64874

Purpose

lentiviral vector with Flag, HA tags

Depositor

Richard Wood

Article

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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