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Showing: 2001 - 2020 of 13516 results

  1. pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

    Plasmid
    #122850
    Purpose
    Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli
    Depositor
    Martin Grininger
    Article
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    m(KS_MAT)_mouseL_ACP (Fasn Mouse)
    Use
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122850-plasmid-map-sequence-id-238544

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  3. pAR69_STRI_m(KS_MAT)_H8_pET22b

    Plasmid
    #122849
    Purpose
    Expresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
    Depositor
    Martin Grininger
    Article
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    m(KS_MAT) (Fasn Mouse)
    Use
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122849-plasmid-map-sequence-id-238542

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  6. pETM6-3F2-mCherry

    Plasmid
    #73418
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 3F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3F2 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73418-plasmid-map-sequence-id-135318

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  11. ymScarletI-YPET pDRF1-GW

    Plasmid
    #168063
    Purpose
    FRET fusion between ymScarletI and YPET for use in yeast
    Depositor
    Bas Teusink
    Insert
    ymScarletI-YPET
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168063-plasmid-map-sequence-id-332368

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  14. pET21-pelB-LaG-2

    Plasmid
    #172739
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-2
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172739-plasmid-map-sequence-id-340192

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  15. pSHDY*in_PpetE:BCD2:mVenus

    Plasmid
    #133970
    Purpose
    Heterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.
    Depositor
    Pia Lindberg
    Article
    Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.
    Insert
    mVenus
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    inserted gene is codon optimized for Synechocyst…
    Promoter
    PpetE:BCD2
    Available since
    April 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133970-plasmid-map-sequence-id-267750

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  20. pET32a-His-FADD

    Plasmid
    #78803
    Purpose
    To purify His-tagged recombinant FADD protein
    Depositor
    Jaewhan Song
    Insert
    GIG3 (FADD Human)
    Use
    Tags
    6x His, S-Tag, Thrombin cleavage site, and TrxA (…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 8, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 2001 - 2020 of 13516 results