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Addgene

CNNM3-CBS-YPet
(Plasmid #149686)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149686 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET-25b-HRV3C-Cter8his
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CNNM3-CBS-YPet
  • Species
    H. sapiens (human), Synthetic
  • Entrez Gene
    CNNM3 (a.k.a. ACDP3)
  • Promoter T7
  • Tag / Fusion Protein
    • 8 histidine tag (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' cloning site: NdeI, 3' cloning site: XhoI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CNNM3-CBS-YPet was a gift from Motoyuki Hattori (Addgene plasmid # 149686 ; http://n2t.net/addgene:149686 ; RRID:Addgene_149686)
  • For your References section:

    A FRET-based screening method to detect potential inhibitors of the binding of CNNM3 to PRL2. Cai F, Huang Y, Wang M, Sun M, Zhao Y, Hattori M. Sci Rep. 2020 Jul 30;10(1):12879. doi: 10.1038/s41598-020-69818-x. 10.1038/s41598-020-69818-x PubMed 32733084