PET28-FnCas12a-EP16
(Plasmid
#183636)
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PurposePET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183636 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePET28
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFnCas12a-EP16
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SpeciesSynthetic
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MutationN607R, K613V, N617R, K180S, K660R, D616N
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Tag
/ Fusion Protein
- 6×His Tag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PET28-FnCas12a-EP16 was a gift from Yunzi Luo (Addgene plasmid # 183636 ; http://n2t.net/addgene:183636 ; RRID:Addgene_183636) -
For your References section:
Improved CRISPR-Cas12a-assisted one-pot DNA editing method enables seamless DNA editing. Wang L, Wang H, Liu H, Zhao Q, Liu B, Wang L, Zhang J, Zhu J, Bao R, Luo Y. Biotechnol Bioeng. 2019 Jun;116(6):1463-1474. doi: 10.1002/bit.26938. Epub 2019 Feb 11. 10.1002/bit.26938 PubMed 30730047
