Showing: 2001 - 2020 of 13889 results
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Plasmid#74054Purposeretroviral expression plasmid for human NFATc2/C (without C-terminal region) with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 AS 1-686 (N terminus deleted), Human)
UseRetroviralTagsAVI-TEVExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterpMSCV-LTRsAvailabilityAcademic Institutions and Nonprofits only -
pSc1
Plasmid#80436PurposeExpresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorInsertssp Cas9 gRNA
eGFP
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
pSc2
Plasmid#80437PurposeExpresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorInsertssp Cas9 gRNA
eGFP
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
pSc1-DD
Plasmid#80439PurposeExpresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorInsertssp Cas9 gRNA
eGFP
UseCRISPRTagsE. coli dihydrofolate reductaseExpressionMammalianMutationPromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
PTRF/Cavin1_L (OZ513)
Plasmid#27188DepositorInsertZinc finger array targeting PTRF/Cavin1 (cavin1b Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PTRF/Cavin1_R (OZ514)
Plasmid#27189DepositorInsertZinc finger array targeting PTRF/Cavin1 (cavin1b Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
NFATc1_L (OZ543)
Plasmid#28068DepositorInsertZinc finger array targeting NFATc1 (nfatc1 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
NFATc1_R (OZ544)
Plasmid#28069DepositorInsertZinc finger array targeting NFATc1 (nfatc1 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
mAzami-Green-Galectin
Plasmid#102419PurposeExpresses mAzami-labeled galectin 3 (Gal3) as a marker for endosomal leakage or rupture.DepositorInsertLGALS3 (LGALS3 Human)
UseTagsmAzami-greenExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5
Plasmid#97044PurposeExpresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.DepositorInsertLigand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
UseLentiviralTagsExpressionMammalianMutationPromoterTet onAvailabilityAcademic Institutions and Nonprofits only -
pBa-LSS-GFP-LDLR wt
Plasmid#98184PurposeLow Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.DepositorInsertLDLR (LDLR Human)
UseTagsGFP and LDLR signal sequence, N-term of GFP so GF…ExpressionMammalianMutationnonePromoterChicken Beta ActinAvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTN-E112A-FL
Plasmid#110218PurposeExpression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsHis6 and ZBExpressionBacterialMutationE112A, P296L (see depositor comments below)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTN-Y117C-FL
Plasmid#110219PurposeExpression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsHis6 and ZBExpressionBacterialMutationY117C, P296L (see depositor comments below)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-CP8B1
Plasmid#191240PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A32
Plasmid#191241PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A31
Plasmid#191242PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc2/C(RIT)-FLAG-AVITEV
Plasmid#74053Purposeretroviral expression plasmid for human NFATc2/C (with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 Human)
UseRetroviralTagsAVI-TEV and FLAGExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterpMSCV-LTRsAvailabilityAcademic Institutions and Nonprofits only -
pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA
Plasmid#85756PurposeAll in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).DepositorInsertsCsy4-T2A-SpRFN-P2A-GFP
gRNA
UseCRISPRTagsCsy4, Csy4 recognition sequence, FokI fusion via …ExpressionMammalianMutationD10A, H840APromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
pSc1-puro
Plasmid#80438PurposeExpresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycinDepositorInsertssp Cas9 gRNA
eGFP
Puromycin resistance
UseCRISPRTagsExpressionMammalianMutationPromoterCMV, U6, and hPGKAvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-SNAPf-DYNC2H1
Plasmid#132524PurposeFull length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragmentsDepositorInsertDYNC2H1 (DYNC2H1 Human)
UseTags8x His, Linker-TEV-linker-TEV, Precission cleavag…ExpressionInsectMutationCodon optimised for Sf9 expressionPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 2001 - 2020 of 13889 results