Addgene

Showing: 1981 - 2000 of 5212 results

Results per page:

Sort results by:

pCAG-TWIN-STREP-FLAG-NPRL2

Plasmid

#164985

Purpose

NPRL2 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

NPRL2 (NPRL2 Human)

Use

Tags

2xSTREP FLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-mCherry-FKBP-GEF(TIAM1)

Plasmid

#85156

Purpose

acute activation of Rac through rapamycin-induced translocation to membrane-localized FRB

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Fusion of mCherry, FKBP, and the DH-domain of human TIAM1 (amino acids 1012-1250)

Use

Tags

mCherry-FKBP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-Z-SpCas9-gRNA-repeat-polyT-11

Plasmid

#197781

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

gRNA repeat for U6 sgRNA assembly

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PET-B-SpCas9-HF1-NLS-6xHis

Plasmid

#207385

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-SpCas9-HF1

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-rat PKA-RIIbeta-mEGFP

Plasmid

#45530

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RII beta tagged by mEGFP

Use

With chicken beta actin promoter and wpre

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-dSpCas9-HF1-VP64-6xHis

Plasmid

#92118

Purpose

Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64

Use

CRISPR

Tags

3xFLAG, 6xHis, NLS, and VP64

Expression

Bacterial

Mutation

D10A, N497A, R661A, Q695A, H840A, Q926A

Promoter

T7

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-Flag-eSpCas9 (without sgRNA)

Plasmid

#92354

Purpose

Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

Cbh

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9

Plasmid

#107858

Purpose

To knock-out TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

gRNA against TARDBP (Exon 2) (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
3xNLS-NLP-cMyc-cMyc enAspCas12a

Plasmid

#182127

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc enAspCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pspCas9-ABE-3'UTR-sgRNA-2xMS2

Plasmid

#132554

Purpose

Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2

Depositor

Baisong Lu

Article

Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

Inserts

ABEmax
sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Flag-Avi-ires-CHD5

Plasmid

#68878

Purpose

Transient mammalian expression of CHD5 for BirA tagging

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

FLAG/AVI

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-mcherry-WIPI2d-cs-TEV-STREP

Plasmid

#178912

Purpose

Mammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d

Use

Tags

C term strep and N term mcherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
3xNLS-NLP-cMyc-cMyc AspCas12a

Plasmid

#182122

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc AspCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCALNL-eGFP-NT1-6-gix-6-NT1

Plasmid

#81204

Purpose

contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

gix-Neo-gix deletion cassette upstream of EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCBa

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Venus-pA_U6-BbsI (pE057)

Plasmid

#149351

Purpose

Mammalian expression vector for Venus

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

Venus

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-EGFP/RFP-miR-E1-1int

Plasmid

#19819

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 . 8():77.

Insert

miRNA

Use

Cre/Lox and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
-161+80pCalb2/E2F2/NRF-1(-64)-like

Plasmid

#66742

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGCG(-64) to TACA

Promoter

Calb2

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-BFP-pA_U6-BbsI (pE058)

Plasmid

#149352

Purpose

Mammalian expression vector for BFP

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS/Daxx myc S502A/669A

Plasmid

#19283

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

Daxx (Daxx Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
M11 Cardiac Actin Promoter pCarA

Plasmid

#17148

Depositor

Randall Moon

Insert

cardiac actin promoter (actc1 Frog)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only