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pCMV-DNAJC5-ΔC40 (Δ151-198)

Plasmid

#205723

Purpose

Mammalian expression of DNAJC5-ΔC40 (Δ151-198)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

Expression

Mammalian

Mutation

Δ151-198

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 321-843 REV1

Plasmid

#201587

Purpose

Expresses the catalytic domain (aa 321-843) of human DNA repair protein REV1 with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA repair protein REV1 (REV1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 321-843 of DNA repair prote…

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23A_delta aa 111-136

Plasmid

#201466

Purpose

Expresses a mutant form of human RAD23A lacking residues 111-136. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 111-136

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23A_delta aa 137-160

Plasmid

#201467

Purpose

Expresses a mutant form of human RAD23A lacking residues 137-160. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 137-160

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23A_delta aa 202-230

Plasmid

#201468

Purpose

Expresses a mutant form of human RAD23A lacking residues 202-230. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 202-230

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23A_delta aa 287-316

Plasmid

#201469

Purpose

Expresses a mutant form of human RAD23A lacking residues 287-316. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 287-316

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-L2D/V9D/I19D PPM1H

Plasmid

#207341

Purpose

Mammalian expression of HA-L2D/V9D/I19D PPM1H

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

PPM1H (PPM1H Human)

Use

Tags

HA

Expression

Mammalian

Mutation

L2D/V9D/I19D

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-NLS-MBP-rATOM(WDR5)

Plasmid

#209710

Purpose

Mammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signal

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

NLS-MBP-rATOM(WDR5)

Use

Tags

NLS-MBP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV Flag-LRRK2 K17A/K18A

Plasmid

#186012

Purpose

Flag-LRRK2 K17A/K18A

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

Flag-LRRK2 K17A/K18A (LRRK2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-BRAF-REG-T241P-Halo

Plasmid

#202549

Purpose

Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag. The REG domain contains the RASopathy mutation, T241P, within its cysteine-rich domain.

Depositor

Deborah Morrison

Article

Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

Insert

BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Threonine 241 mutated to proline

Promoter

CMV

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta55-118-10xHis

Plasmid

#194180

Purpose

Expresses MPP8-delta55-118_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta55-118 (chromo domain deletion)

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta2-441-10xHis

Plasmid

#194181

Purpose

Expresses MPP8-delta2-441_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta2-441

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta442-860-10xHis

Plasmid

#194182

Purpose

Expresses MPP8-delta442-860_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta442-860

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta600-728-10xHis

Plasmid

#194183

Purpose

Expresses MPP8-delta600-728_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta600-728 (4xANK deletion)

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagC-PrP-SBP-HsNot10_AH

Plasmid

#148844

Purpose

Mammalian Expression of HsNot10

Depositor

Elisa Izaurralde

Insert

HsNot10 (CNOT10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagC-PrP-SBP-HsNot11_AH

Plasmid

#148845

Purpose

Mammalian Expression of HsNot11

Depositor

Elisa Izaurralde

Insert

HsNot11 (CNOT11 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagN-PrP-SBP-HsAGO2_AC

Plasmid

#148440

Purpose

Mammalian Expression of HsAGO2

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsAGO2 (AGO2 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation C840Tand sequence differs in …

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagN-PrP-SBP-EGFP_W

Plasmid

#147820

Purpose

Mammalian Expression of EGFP

Depositor

Elisa Izaurralde

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry-EPHX1c.337T>C-Flag

Plasmid

#169169

Purpose

Expresses EPHX1 c.337T>C in mammalian cells

Depositor

Bruno Feve, Jeremie Gautheron

Article

Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

Insert

epoxide hydrolase 1 (EPHX1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

c.337T>C

Promoter

CMV

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry-EPHX1c.416A>G-Flag

Plasmid

#169170

Purpose

Expresses EPHX1 c.416A>G in mammalian cells

Depositor

Bruno Feve, Jeremie Gautheron

Article

Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

Insert

epoxide hydrolase 1 (EPHX1 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

c.337T>C

Promoter

CMV

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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