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Showing: 4951 - 5000 of 156661 results

  6. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60229
    Purpose
    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60229-plasmid-map-sequence-id-419029

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  13. MAPK3

    Plasmid
    #73235
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAPK3 (MAPK3 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00069:Pkinase 42-330
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73235-plasmid-map-sequence-id-137225

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  17. pTRIPZ(M)-HT-Cbx8

    Plasmid
    #82516
    Purpose
    Lentivirus vector. Expresses halotag Cbx8fusion proteins in mammalian cells.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 8 (CBX8 Human)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82516-plasmid-map-sequence-id-156792

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  20. mTagRFP-Membrane-1

    Plasmid
    #57992
    Purpose
    Localization: Plasma Membrane, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    N-terminal 20 Aas MLS from Neuromodulin and mTagR…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    57992-plasmid-map-sequence-id-313444

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  21. pM-Cas9

    Plasmid
    #196325
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding SpCas9 in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196325-plasmid-map-sequence-id-390015

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  23. pHflu4

    Plasmid
    #203882
    Purpose
    pHI1 with insertion of monomeric red fluorescent protein (mRFP)
    Depositor
    Bogumil Karas
    Insert
    monomeric red fluorescent protein
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    203882-plasmid-map-sequence-id-403835

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  28. TFORF2904

    Plasmid
    #143388
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DACH1 (DACH1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143388-plasmid-map-sequence-id-400329

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  31. pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G

    Plasmid
    #194458
    Purpose
    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.
    Depositor
    Ana Sofia Coroadinha
    Article
    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:
    Insert
    Anti-CD19 CAR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    194458-plasmid-map-sequence-id-384169

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  39. pLEW100v5

    Plasmid
    #24011
    Depositor
    George Cross
    Insert
    Luciferase
    Use
    Trypanosome expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 25, 2010
    Availability
    Academic Institutions and Nonprofits only
    24011-plasmid-map-sequence-id-11282

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  42. pOP5GP

    Plasmid
    #112610
    Purpose
    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-GST and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112610-plasmid-map-sequence-id-220240

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  44. AAV-sCAG-mBaoJin-oPRE

    Plasmid
    #218684
    Purpose
    AAV vector plasmid expressing mBaoJin under a short variant of the CAG (sCAG) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.
    Depositor
    Bart Nieuwenhuis
    Insert
    mBaoJin
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Short variant of the CAG promoter
    Available since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    218684-plasmid-map-sequence-id-438234

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  45. pLIX403-EBFP2-ccdB-NatMx

    Plasmid
    #158559
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158559-plasmid-map-sequence-id-309875

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Showing: 4951 - 5000 of 156661 results