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Addgene

pMRX-IB-HaloTag7-mGFP
(Plasmid #184903)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184903 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMRX-IB
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    monomeric EGFP
  • Insert Size (bp)
    717
  • Tag / Fusion Protein
    • HaloTag7 (N terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRX-IB-HaloTag7-mGFP was a gift from Noboru Mizushima (Addgene plasmid # 184903 ; http://n2t.net/addgene:184903 ; RRID:Addgene_184903)
  • For your References section:

    A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Yim WW, Yamamoto H, Mizushima N. Elife. 2022 Aug 8;11. pii: 78923. doi: 10.7554/eLife.78923. 10.7554/eLife.78923 PubMed 35938926