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Plasmid#15782DepositorInsertUbiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
UseTags6x His, T7 tag, and Xpress tag (enterokinase cleaā¦ExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1-T17N
Plasmid#12982DepositorInsertRac1 DN (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationT17N Dominant negativePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBADHISTEV_PfeE
Plasmid#129120PurposepBADHISTEV carrying pfeE for overexpression of PfeE in E. coliDepositorInsertPeriplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationSignal peptide sequence has been removedPromoterPBADAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-PC-GCaMP6f
Plasmid#73503PurposeGCaMP6f knock in construct into the AAVS1 locusDepositorInsertGCaMP6f
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV Puro DEST ERKKTRClover
Plasmid#59150PurposeLentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-MCS
Plasmid#84008Purposedoxycycline-inducible lentiviral vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterTRE-CMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro-hTERT
Plasmid#1773PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorInserthTERT (TERT Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4 myc PGC-1 alpha
Plasmid#10974PurposeMammalian expression of PGC-1a with myc and his tagsDepositorInsertPGC-1 alpha (PPARGC1A Human)
UseTagsHis and MycExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pRL443
Plasmid#70261PurposeShuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-tetO-hNIL
Plasmid#197089PurposePiggyBac plasmid with tet-inducible expression of transcription factors for motor neuron differentiationDepositorUseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV mGAD65(delE1) GFP WPRE SV40pA
Plasmid#177316PurposeTo produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.DepositorInsertmGAD65(delE1) promoter, GABAergic interneurons specific promoter
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Foxp1A
Plasmid#16362DepositorInsertFOXP1A (Foxp1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1346 U6-B2M sgRNA Gag-pol v2
Plasmid#201917PurposeCas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5ā-GAGTAGCGCGAGCACAGCTA)DepositorInsertsGag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
UseTagsExpressionMammalianMutationPromoterCAG and Human U6Available sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-Ī±-chain
Plasmid#133730PurposeExpresses the gePSI-Ī±-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.DepositorInsertsa-Chain_gePSI
ZsGreen1
UseTagsExpressionMammalianMutationOnly one part of the gePSI-system (aa1-141 + stopā¦PromoterpTRE3G-BiAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-L11
Plasmid#20936DepositorInsertRibosomal protein L11 (RPL11 Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLVCT-rtTR-KRAB-2SM2
Plasmid#11779PurposeTet-regulated (Tet-off) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertCAG promoters, GFP, rtTR-KRAB-2SM2, Tet-off
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_FLAG-BPTF
Plasmid#102242PurposeExpression of human Flag-BPTF using baculovirus from pFastBac1DepositorInsertFlag-BPTF (BPTF Human)
UseTagsFlagExpressionInsectMutationPromoterPolyhedronAvailable sinceDec. 11, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
SPDK3876(TRV-RNA2-pPEBV-MCS)
Plasmid#149275PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAsDepositorInsertModified TRV RNA2 with PEBV promoter
UseT-dna vectorTagsExpressionMutationT2318C (DNA) in PEBV coat protein sequencePromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG mFoxg1-GFP wildtype
Plasmid#73048PurposeC-terminal fusion of gfp Mouse wildtype Foxg1DepositorInsertmFoxg1 (Foxg1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMMP-BiP-IRES-mRFP
Plasmid#36975DepositorInsertBiP (HSPA5 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
MX2_pCSdest
Plasmid#53942DepositorInsertMX2 (MX2 Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-nEF Con/Fon DREADD Gq-mCherry
Plasmid#183532PurposeINTRSECT/ChemogeneticsDepositorInsertDREADD Gq-mCherry
UseAAVTagsExpressionMutationNonePromoternEFAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWPT-/GCCACC-mEGFP-IRES-mCherry
Plasmid#49235PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0
Plasmid#149662PurposeConjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.DepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1
Plasmid#193942Purposea fully murine CAR targeting CD105 (endoglin), a component of the TGFĪ² receptor expressed on the surface of certain solid tumors and acute leukemias.DepositorInsertheavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_BA.2.86
Plasmid#208441Purposeexpressing SARS-CoV-2 BA.2.86 spike protein for pseudovirus productionDepositorInsertSpike (BA.2.86) (S SARS-CoV-2)
UseTagsExpressionMammalianMutationsee GISAID sequencePromoterCMVAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICSL01009::AtU6p
Plasmid#46968PurposeEncodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.DepositorInsertAtU6p
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMVĪ²-p300-myc
Plasmid#30489PurposeMammalian expression of human p300 with myc tagDepositorInsertp300 (EP300 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFP-CD63
Plasmid#194989Purposeoverexpress CD63DepositorInsertGFP-CD63
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1
Plasmid#85069PurposeYellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)DepositorInsertPH-PLCD1 (PLCD1 Human)
UseTagsIRES-sYFP2-PH and mScarletExpressionMammalianMutationPLCD aa 1ā170PromoterCMVAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
TagRFP-T_A1aY1
Plasmid#158751PurposeLentiviral vector for expression of TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.DepositorInsertTagRFP-T_A1aY1
UseLentiviralTagsTagRFP-TExpressionMammalianMutationPromoterCAG (CMV enhancer and chicken beta actin promoter)Available sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-UbC-rtTA-Ngn2:2A:EGFP
Plasmid#127288Purposeall-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)DepositorInsertNgn2:2A:Ascl1 (NEUROG2 Human)
UseLentiviralTagsNgn2 and EGFP linked via 2AExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTALYM3B15
Plasmid#47878PurposeTALE-mClover against mouse major satellite sequenceDepositorInsertTALE-mClover
UseTALENTagsmCloverExpressionMammalianMutationPromoterCAGAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsXRN1
Plasmid#66596Purposeexpresses lambdaN-HA-tagged XRN1 in mammalian cellsDepositorInsertXRN1 (XRN1 Human)
UseTagslambdaN-HAExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15-MHL
Plasmid#26092PurposeSGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.DepositorTypeEmpty backboneUseTagsHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceSept. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
scFLARE2
Plasmid#158700PurposeNeuronal expression of scFLARE2 componentDepositorInsertscFLARE2
UseAAVTagsHA, Flag, VP16ExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aciā¦PromoterSynAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
p16sheLL
Plasmid#37320DepositorInsertHPV16L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
CN3572-rAAV-3xcore2(390h)-minBglobin-SYFP2-WPRE3-BGHpA
Plasmid#208143PurposeDirect-expressing SYFP2 in astrocytes AAV VirusInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterBeta Globin minimal promoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-14-3-3 beta
Plasmid#13270DepositorInsert14-3-3 beta (YWHAB Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only