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Plasmid#119573PurposeLevel 0 part. PromoterDepositorInsertPcoaT
UseSynthetic BiologyTagsExpressionMutationRBS* added (22 bp) upstream of ATG.PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNB2629
Plasmid#113394PurposeMET25p-MS2-RFP-CYC1 TTDepositorInsertMS2 bacteriophage coat protein
UseTagsred fluorescent protein (RFP), codon-optimized fo…ExpressionYeastMutationPromoterAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)
Plasmid#219537Purposea. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotorDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE-UBC-MCP-mNeonGreen
Plasmid#207581PurposeThe lentiviral plasmids for expression of MCP-NeonGreenDepositorInsertMS2 coat protein mNeonGreen fusion
UseLentiviralTagsmNeonGreenExpressionMutationPromoterUBCAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYI001
Plasmid#170833Purpose(GUSPlus::tOCS in pFP100)*DepositorInsertGUSPlus::tOCS
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC037-HisMS2_PLP_pac
Plasmid#128233PurposeExpression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.DepositorInsertsMaturation Protein
Coat Protein Dimer
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCu-MCP-2yeGFP(405)
Plasmid#121419PurposeExpressing MCP-2yeGFPDepositorInsertCUP1p-MCP-2yeGFP
UseTagsExpressionYeastMutationPromoterCUP1 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP
Plasmid#183934PurposePCP tandem dimer binding to PP7 RNA stem loops; labeled with GFPDepositorInserttdPCP-GFP
UseTagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-amidase
Plasmid#128135PurposeThe cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertamidase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMS2CP(V29I)-myc-T2A-tagRFP
Plasmid#140260PurposeExpresses MS2CP(V29I) in mammalian cellsDepositorInsertCoat protein of bacteriphage MS2
UseSynthetic BiologyTagsmyc-T2A-tagRFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMC051-HisMS2_PLP_pac_LC
Plasmid#128234PurposeLower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.DepositorInsertsMaturation Protein
Coat Protein Dimer
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
plIVMD156
Plasmid#160934PurposeIn vivo mRNA display Gateway Destination constructDepositorInsertMCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop
UseTagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastMutationPromoterMET25Available sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3_pBen-sfGFP_J23101-BenR
Plasmid#128125PurposeExpressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032DepositorInsertsfGFP and BenR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBen and J23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
plIVMD164
Plasmid#160936PurposeIn vivo mRNA display construct for GFPDepositorInsertMCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop
UseTagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastMutationPromoterMET25Available sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB4C5_J23101-CocE
Plasmid#128129PurposeExpressing CocE enzyme (cocaine to benzoate) gene under control of the constitutive promoter J23101, and RBS B0032DepositorInsertCocE
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
MCP-YPet
Plasmid#215747PurposeExpresses MS2 coat protein (MCP) fused to YPetDepositorInsertYPet
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
CTAG_eGFP-HDEL
Plasmid#136097PurposeFP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptideDepositorInsertCTAG_eGFP(noATG,noStop)-HDEL
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CTAG_mTurquoise2-HDEL
Plasmid#136105PurposeFP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptideDepositorInsertCTAG_mTurquoise2(noATG,noStop)-HDEL
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CTAG_mVenus-HDEL
Plasmid#136107PurposeFP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptideDepositorInsertCTAG_mVenus(noATG,noStop)-HDEL
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
plIVMD163
Plasmid#160935PurposeIn vivo mRNA display construct for mCherryDepositorInsertMCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop
UseTagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastMutationPromoterMET25Available sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB4C5_J23101-vdh
Plasmid#128130PurposeExpressing vdh gene (for the enzyme transforming benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertvdh
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-HipO
Plasmid#128133PurposeThe cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertHipO
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
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phage ubc nls ha 2xmcp HALO
Plasmid#64540PurposeUsed to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.DepositorInsertMCP
UseLentiviralTagsHA, HALO, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
phage UBC NLS-HA-2XMCP-tagRFPt
Plasmid#64541PurposeUsed to label reporter mRNAs. Lentiviral expression of the MCP protein fused to tagRFP.DepositorInsertMCP
UseLentiviralTagsHA, NLS, and tagRFP-TExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMALcPP-MS2cp
Plasmid#203351PurposeExpresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteriaDepositorInsertMS2 coat protein
UseTagsHx6-MBPExpressionBacterialMutationV75E and A81G, to prevent capsid assemblyPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
MCP-mCherry-TIAL1
Plasmid#207669PurposeTo express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIAL1DepositorInsertTIAL1 (TIAL1 Human)
UseTagsMCP-mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-HA-tdMCP-tdTomato
Plasmid#183938Purposetandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tagDepositorInserttdMCP-tdTomato (cp Synthetic)
UseTagsNLS-HAExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJRTCLCrVA.008
Plasmid#22841DepositorInsertA DNA (with MCS)
UseCotton vigs vectorTagsExpressionBacterialMutationCoat Protein (AR1 gene) of Cotton Leaf Crumple Vi…PromoterAvailable sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRW006-SM-destination-pro35S-Cas9
Plasmid#104439PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoter35SAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-COPE
Plasmid#31585DepositorInsertcoatomer protein complex, subunit epsilon (COPE Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP-VPR
Plasmid#183936PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFPDepositorInserttdPCP-GFP-VPR
UseTagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-QCP-3xBFP
Plasmid#158206PurposeQb coat protein fused to three repeats of the blue fluorescent protein under a Efla promoter.DepositorInsertQCP under the Elfa promoter and three BFP tags
UseSynthetic BiologyTags3xBFPExpressionMammalianMutationPromoterElfaAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
MCP-mCherry-TIS11B
Plasmid#207670PurposeTo express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIS11BDepositorInsertTIS11B (ZFP36L1 Human)
UseTagsMCP-mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYI006
Plasmid#170840PurposeFRO6p::GUSPlus::tOCS in pFP100DepositorInsertFRO6p::GUSPlus::tOCS
UseTagsExpressionPlantMutationPromoterFRO6Available sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
CTAG_eGFP-GHtag-HDEL
Plasmid#136096PurposeFP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tagDepositorInsertCTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
XXXXA-pHG165c
Plasmid#170110PurposeConstitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.DepositorInsertXXXXA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
XXXXA_Q182A_R287H-pHG165c
Plasmid#170111PurposeConstitutively expressed , mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.DepositorInsertXXXXA_Q182A_R278H
UseTagsExpressionBacterialMutationQ182A, R287HPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xQb oligo-library based
Plasmid#158201PurposeCassette of 10 different binding sites with high affinity to QB phage coat protein, as extracted from the oligo pool experiment.DepositorInsert10 binding sites of QB based on oligo library experiment
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRWJB006
Plasmid#160113PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoter35SAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRWJB004
Plasmid#160112PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoterUBQ10Available sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-vdh
Plasmid#128134PurposeThe cell-free adapted backbone, pBEAST, expressing vdh (the enzyme converting benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertvdh
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK287
Plasmid#71120PurposeProduces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (H6UctB) in E. coliDepositorInsertformyl-CoA:oxalate CoA-transferase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCP-Luc (MCP-1 promoter in pGL3-basic)
Plasmid#40324DepositorInsertMCP-1 promoter (Ccl2 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationcontains MCP-1 promoter region through the nucleo…PromoterMCP-1Available sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2CP-VPg(FCV)
Plasmid#167314PurposeTemplate pDNA to prepare CaVT mRNADepositorInsertCaVT
UseSynthetic BiologyTagsDYK-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG_PCP-T5Exo
Plasmid#214736Purposeencodes T5 exonuclease with a PP7 coat protein fused to the N terminusDepositorInsertCAG_PCP-T5Exo_bGH
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
StrKDEL-IRES_mScarleti_Sec23A
Plasmid#117273PurposeExpresses an Str-KDEL Hook for the RUSH system and separately mScarleti-Sec23ADepositorInsertsUseTagsmScarlet-iExpressionMammalianMutationHuman Sec23A; Sequencing showed a mutation in Sec…PromoterCMV and IRESAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG MCP-VP64
Plasmid#92357PurposeCAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.DepositorInsertMCP-VP64
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRW004-SM-destination-proUBQ10-Cas9
Plasmid#104438PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoterUBQ10Available sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-NbMS2
Plasmid#171701PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the coat protein of bacteriophage MS2 via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only