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Plasmid#84268PurposeA versatile vector for recombinant mycobacterial protein productionDepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterAceteamidaseAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only
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pMyCA-mCherry
Plasmid#84272PurposemCherry fluorescence protein expression using M. smegmatisDepositorInsertmCherry
UseTagsHis6ExpressionBacterialMutationPromoterAcetamidaseAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS4.1w-tet-on-NORAD
Plasmid#125010Purposeexpress in mammalian cellDepositorInsertHomo sapiens non-coding RNA activated by DNA damage (NORAD), long non-coding RNA (NORAD Human)
UseTagsExpressionMammalianMutationPromoterTRE-TightAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
RK5F-YAP8SA
Plasmid#125008Purposeexpress in mammalian cellDepositorInsertYAP8SA (YAP1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLAS3W-S100P
Plasmid#125009Purposeexpress in mammalian cellDepositorInsertS100P (S100P Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-CES2A-ΔC-S227A
Plasmid#200556PurposeMammalian expression of secreted mouse CES2A S227A mutantDepositorInsertMouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)
UseTags6xHis, Flag, and V5ExpressionMammalianMutationPromoterCMV promoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-CES2A-ΔC
Plasmid#200554PurposeMammalian expression of secreted mouse CES2ADepositorInsertMouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)
UseTags6xHis, Flag, and V5ExpressionMammalianMutationPromoterCMV promoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Tbg-CES2A-ΔC
Plasmid#200558PurposeSecreted mouse CES2A driven by heptaocyte-specific promoter in AAV viral vectorDepositorInsertMouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)
UseAAVTagsFlagExpressionMutationPromoterTBG promoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-CES2C-ΔC
Plasmid#200555PurposeMammalian expression of secreted mouse CES2CDepositorInsertMouse Carboxylesterase 2C delta C-terminal (Ces2c Mouse)
UseTags6xHis, Flag, and V5ExpressionMammalianMutationPromoterCMV promoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-CES2C-ΔC-S230A
Plasmid#200557PurposeMammalian expression of secreted mouse CES2C S230A mutantDepositorInsertMouse Carboxylesterase 2C S230A mutant delta C-terminal (Ces2c Mouse)
UseTags6xHis, Flag, and V5ExpressionMammalianMutationPromoterCMV promoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Tbg-CES2A-ΔC-S227A
Plasmid#200560PurposeSecreted mouse CES2A S227A mutant driven by heptaocyte-specific promoter in AAV viral vectorDepositorInsertMouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)
UseAAVTagsFlagExpressionMutationPromoterCMV promoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Tbg-CES2C-ΔC
Plasmid#200559PurposeSecreted mouse CES2C driven by heptaocyte-specific promoter in AAV viral vectorDepositorInsertMouse Carboxylesterase 2C delta C-terminal (Ces2c Mouse)
UseAAVTagsFlagExpressionMutationPromoterTBG promoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Tbg-CES2C-ΔC-S230A
Plasmid#200561PurposeSecreted mouse CES2C S230A mutant driven by heptaocyte-specific promoter in AAV viral vectorDepositorInsertMouse Carboxylesterase 2C S230A mutant delta C-terminal (Ces2c Mouse)
UseAAVTagsFlagExpressionMutationPromoterTBG promoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
2xFKBP-mCh-KDEL
Plasmid#173010PurposeFKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.DepositorInsert2xFKBP-mCh-KDEL
UseTagsmCherryExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
TfR-mCh-DHFR
Plasmid#173013PurposeFor light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.DepositorInsertTfR-mCh-DHFR
UseTagsDHFR and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-NL1
Plasmid#173007PurposeFor light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.DepositorInsertDHFR-GFP-NL1
UseTagsDHFR and EGFPExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-GluA1
Plasmid#173008PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.DepositorInsertDHFR-GFP-th-GluA1
UseLentiviralTagsGFPExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
DHFR-mNeon-GluA1
Plasmid#173009PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.DepositorInsertDHFR-mNeon-GluA1
UseTagsDHFR and mNeonExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
TfR-moxGFP-DHFR
Plasmid#173012PurposeFor light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).DepositorInsertTfR-moxGFP-DHFR
UseTagsDHFR and moxGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
2xFKBP-smFP(myc)-KDEL
Plasmid#173021PurposeFKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.DepositorInsert2xFKBP-smFP(myc)-KDEL
UseTagsFKBP and smFP(myc) non-fluorescentExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only