pDEST40-CES2A-ΔC-S227A
(Plasmid #200556)

• Purpose: Mammalian expression of secreted mouse CES2A S227A mutant
• Depositing Lab: Jonathan Long
• Publication: Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00138-9. doi: 10.1016/j.cmet.2023.04.011.

Backbone

• Vector backbone: pDEST40
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mouse Carboxylesterase 2A S227A mutant delta C-terminal
• Species: M. musculus (mouse)
• Entrez Gene: Ces2a (a.k.a. 9130231C15Rik, Ces6)
• Promoter: CMV promoter
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • 6xHis (C terminal on insert)
  • V5
  • 6xHis

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pDEST40-CES2A S227A-delta C.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry