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Plasmid#113492PurposeExpression of human Cezanne with C-terminal strep-HA tagDepositorInsertCezanne (OTUD7B Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-Cezanne (OTU, aa 53-446)
Plasmid#61581PurposeExpresses human Cezanne (OTU domain) in E. coli.DepositorInsertCezanne (OTUD7B Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-52 and aa 447-843.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-Cezanne2 (OTU+UBA, aa 1-462)
Plasmid#61582PurposeExpresses human Cezanne2 (OTU+UBA domains) in E. coli.DepositorInsertCezanne2 (OTUD7A Human)
UseTagsHis6-GST-3CExpressionBacterialMutationIsoform 2. Deleted aa 463-933.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pluc2-iRFP720
Plasmid#98887PurposeMammalian expressionDepositorInsertFirefly Luciferase
UseTagsiRFP720ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-TWIST2
Plasmid#197935PurposeGateway entry vector for an inducible 3xFLAG-tagged TWIST2DepositorInsertTWIST2 (TWIST2 Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
FEMP
Plasmid#191973PurposeFRET-based indicator of ER-mitochondria proximity (FEMP)DepositorInsertAkapYFP-Tav2a-CFP-sac1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-TWIST2 neo
Plasmid#197936PurposeLentiviral expression vector for an inducible 3xFLAG-tagged TWIST2DepositorInsertTWIST2 (TWIST2 Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAurora2
Plasmid#213134PurposeIntegrated transcriptional and translational control for stringently blue-light-activated gene expressionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRoRL
Plasmid#213132PurposeRiboptoregulator circuit RoRL for blue-light-activated gene expressionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRoRH
Plasmid#213133PurposeRiboptoregulator circuit RoRH for blue-light-activated gene expressionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
LI D-E TurboID
Plasmid#209386PurposeGolden Gate LI D-E moduleDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationTurboID gene sequence C249APromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
LI D-E TurboID-GFP
Plasmid#209387PurposeGolden Gate LI D-E moduleDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationTurboID gene sequence C249APromoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
LI B-C TurboID
Plasmid#209385PurposeGolden Gate LI B-C moduleDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationTurboID gene sequence C249APromoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
recoverin1b
Plasmid#83812Purposeexpress Zebrafish recoverin1bDepositorInsertrecoverin1b (rcvrnb Zebrafish)
UseTagsExpressionBacterialMutation*(see below)PromoterAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
recoverin2b
Plasmid#83815Purposeexpress zebrafish recoverin2bDepositorInsertrecoverin2b (rcvrn3 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Xt-VSP
Plasmid#51887Purposeexpression of Xt-VSP in Xenopus oocytes or mammalian cellsDepositorInsertVSP
UseXenopus oocyte expressionTagsExpressionMammalianMutationsee comments*PromoterCMVAvailable sinceApril 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
recoverin1a
Plasmid#83811Purposeexpress zebrafish recoverin1aDepositorInsertrecoverin1a (rcvrna Zebrafish)
UseTagsExpressionBacterialMutation*(see below)PromoterAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
recoverin2a
Plasmid#83813Purposeexpress zebrafish recoverin2aDepositorInsertrecoverin2a (rcvrn2 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-Xl-VSP2
Plasmid#51885Purposeexpression of Xl-VSP2 in Xenopus oocytes or mammalian cellsDepositorInsertVSP2
UseXenopus oocyte expressionTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only