Addgene

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pRS416 Gal-RNQ1 153-405-YFP

Plasmid

#18689

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 153-405

Use

Tags

YFP

Expression

Yeast

Mutation

Prion domain of Rnq1

Promoter

Availability

Academic Institutions and Nonprofits only
pGAN101

Plasmid

#131123

Purpose

Plasmid for overexpression of prion domains to induce prions

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Tags

mKate2

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Efg1-ΔDBD

Plasmid

#216396

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 182-356

Promoter

Availability

Academic Institutions and Nonprofits only

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Efg1-N-PrLD

Plasmid

#216397

Purpose

Expresses 6xHis-tagged N-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-N-PrLD

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 182-554

Promoter

Availability

Academic Institutions and Nonprofits only

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Efg1-C-PrLD

Plasmid

#216398

Purpose

Expresses 6xHis-tagged C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-C-PrLD

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Q443P

Plasmid

#216401

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutation

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-Q443P

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Glutamine 443 to Proline, and deleted ami…

Promoter

Availability

Academic Institutions and Nonprofits only

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mSecPrP-pCB6+

Plasmid

#13917

Depositor

Susan Lindquist

Article

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP (Prnp Mouse)

Use

Tags

Expression

Mammalian

Mutation

3F4 epitag

Promoter

Availability

Academic Institutions and Nonprofits only
Efg1-ΔDBD-A51P/A56P

Plasmid

#216400

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56 to proline mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-A51P/A56P

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51 and 56 to Proline, and deleted…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416 Gal-RNQ1 1-153-YFP

Plasmid

#18690

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 1-153

Use

Tags

YFP

Expression

Yeast

Mutation

NonPrion domain of Rnq1

Promoter

Availability

Academic Institutions and Nonprofits only
moPrP(3F4) D177N

Plasmid

#1319

Depositor

Susan Lindquist

Article

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP D177N (Prnp Mouse)

Use

Tags

Expression

Mammalian

Mutation

D177N point mutation

Promoter

Availability

Academic Institutions and Nonprofits only
Efg1-ΔDBD-A51P/A56P/Q443P

Plasmid

#216402

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q443 to proline mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-A51P/A56P/Q443P

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51, 56 and Glutamine 443 to Proli…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Efg1-ΔDBD-A51P/A56P/Q70P/Q443P

Plasmid

#216403

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/Q443 to proline mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-A51P/A56P/Q70P/Q443P

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51, 56 and Glutamine 70, 443 to P…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Efg1-ΔDBD-Y4S/Y8S/Y16S

Plasmid

#216405

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y4/Y8/Y16 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-Y4S/Y8S/Y16S

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 4, 8 and 16 to Serine, and delet…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Efg1-ΔDBD-Y64S/Y66S/Y69S

Plasmid

#216406

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y64/Y66/Y69 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-Y64S/Y66S/Y69S

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 64, 66 and 69 to Serine, and del…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Efg1-ΔDBD-Y101S/Y104S/Y106S

Plasmid

#216407

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y101/Y104/Y106 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-Y101S/Y104S/Y106S

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 101, 104 and 106 to Serine, and …

Promoter

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Y115S/Y132S/Y139S

Plasmid

#216408

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y115/Y132/Y139 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-Y115S/Y132S/Y139S

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 115, 132 and 139 to Serine, and …

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Efg1-ΔDBD-Y401S/Y404S/Y409S

Plasmid

#216409

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y401/Y404/Y409 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-Y401S/Y404S/Y409S

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 401, 404 and 409 to Serine, and …

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Efg1-ΔDBD-Y450S/Y452S/Y456S

Plasmid

#216410

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y450/Y452/Y456 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-Y450S/Y452S/Y456S

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 450, 452 and 456 to Serine, and …

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Efg1-ΔDBD-Y478S/Y482S/Y484S

Plasmid

#216411

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y478/Y482/Y484 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-Y478S/Y482S/Y484S

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 478, 482 and 484 to Serine, and …

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P

Plasmid

#216404

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/N102/Q443 to proline mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51, 56, Glutamine 70, 443 and Asp…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge