moPrP(3F4) D177N
(Plasmid
#1319)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 1319 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcb6
- Backbone size w/o insert (bp) 7200
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePrP D177N
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Alt nameprion protein
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SpeciesM. musculus (mouse)
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Insert Size (bp)900
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MutationD177N point mutation
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GenBank IDNM_011170
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Entrez GenePrnp (a.k.a. CD230, PrP, PrP<C>, PrPC, PrPSc, Prn-i, Prn-p, Sinc, prP27-30, prP33-35C)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site bglII, EcoRI, KpnI, NotI, ClaI, EcoRV (not destroyed)
- 3′ cloning site bamHI??? (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
moPrP(3F4) D177N was a gift from Susan Lindquist (Addgene plasmid # 1319 ; http://n2t.net/addgene:1319 ; RRID:Addgene_1319) -
For your References section:
Wild-type PrP and a mutant associated with prion disease are subject to retrograde transport and proteasome degradation. Ma J, Lindquist S. Proc Natl Acad Sci U S A 2001 Dec 18;98(26):14955-60. Epub 2001 Dec 11. 10.1073/pnas.011578098 PubMed 11742063