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Plasmid#44899PurposeYeast tagging vector to create gene fusion with yeast optimized mTagBFP2 with Kan selectionDepositorInsertyomTagBFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKT0127
Plasmid#8728DepositorInsertyEGFP
UseTagsCodon optimized linkerExpressionYeastMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAV10.KH
Plasmid#63208PurposepCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWS3865
Plasmid#185742PurposeSub-array backbone 1/4DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS3866
Plasmid#185743PurposeSub-array backbone 2/4DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS3867
Plasmid#185744PurposeSub-array backbone 3/4DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2
Plasmid#171104PurposeExpresses sgRNA (ENO1 gRNA) in Candida auris.DepositorInsertsgRNA
UseTagsExpressionYeastMutationPromoterADH1Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
p4A8_S7T_BC
Plasmid#217832Purpose4A8 variant in barcoded, yeast surface display plasmidDepositorInsert4A8 variant Fab
UseTagsExpressionYeastMutationVL: S7TPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWS3868
Plasmid#185745PurposeSub-array backbone 4/4DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP_LEU2D
Plasmid#58352PurposeUsed for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiaeDepositorTypeEmpty backboneUseTagsGFP-HisExpressionYeastMutationPromoterGAL1Available sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTO135_pENO1-CaCas9-tCyc1
Plasmid#171103PurposeExpresses Cas9 in Candida aurisDepositorInsertCaCas9
UseTagsExpressionYeastMutationPromoterENO1Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYZ462
Plasmid#187970PurposeTEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4tDepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF1Available sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH-tdGFP
Plasmid#74322PurposepH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiaeDepositorInsertpH-tdGFP
UseYeast taggingTagsnoneExpressionYeastMutationN149Y, Q204HPromoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNTI661 pRPR1(TetO)-sgRNA
Plasmid#139475PurposeVector for tet-regulated sgRNA expression in yeastDepositorInsertsingle guide RNA scaffold
UseCRISPRTagsExpressionYeastMutationPromoterpRPR1(TetO)Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTH733-2µ-RLuc/maxCFLuc
Plasmid#40608DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFG2
Plasmid#87250Purposegene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-NatDepositorInsertNat
UseTagsparS segmentsExpressionYeastMutationPromoterTEFAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB3cH_pGAP_23*_pLAT1_Cas9
Plasmid#104907PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on HygDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGal4AD-CIBN
Plasmid#28246PurposeExpresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activationDepositorInsertCIB1 (1-171aa)
UseTagsGal4ADExpressionYeastMutationPromoterAvailable sinceMarch 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS712
Plasmid#213041PurposeCRISPR activation based overexpression in Nakaseomyces (Candida) glabrataDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pABY-c45
Plasmid#201771PurposeExpresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterTEF1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only