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YEp195-CUP1-His-Smt3

Plasmid

#99538

Purpose

Yeast episomal vector for expression of His-tagged Smt3 (SUMO); URA3 marker

Depositor

Helle Ulrich

Article

Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

Insert

SMT3 (SMT3 Budding Yeast)

Use

Tags

7xHis

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-PmCDA1

Plasmid

#60818

Purpose

to overexpress lamprey deaminase in yeast

Depositor

Youri Pavlov

Article

Lada et al PLoS Genet. 2013;9(9):e1003736. doi: 10.

Insert

CDA1

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

GAL10

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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RPL25-GFP (94)

Plasmid

#24037

Depositor

Michael Rout

Insert

RPL25 (RPL25 Budding Yeast)

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2010

Availability

Academic Institutions and Nonprofits only
pCfB8379

Plasmid

#126913

Purpose

Plasmid containing integration cassette at X-4 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and XdCrtYB

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and XdCrtYB

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a- mCFP-kanMX6

Plasmid

#105145

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mCFP-kanMX6

Use

Tags

mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pESC-URA-Ub-G76A-Rnq1-GFP

Plasmid

#21052

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

Rnq1 (RNQ1 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP and ubiquitin

Expression

Yeast

Mutation

Promoter

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pTH671-SUP35-H348Q

Plasmid

#29732

Depositor

Tobias von der Haar

Insert

SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

H348Q mutant gene of SUP35 including genomic sequ…

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pILGFP97

Plasmid

#83551

Purpose

Used to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

CYC1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

CYC1

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB8381

Plasmid

#126915

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and tHMG1

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and tHMG1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pJK148-Pnmt41-GBP-mCherry(C)-leu1+

Plasmid

#89069

Purpose

genomic GBP-mCherry epitope C-terminal tagging at leu1+ locus in S. pombe

Depositor

Quanwen Jin

Article

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Genomic integration in s. pombe

Tags

GBP-mCherry

Expression

Yeast

Mutation

Promoter

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-mEOS3.2-KanMX6

Plasmid

#87030

Purpose

Tag S. pombe genes in C terminal with mEOS3.2

Depositor

Julien Berro, Thomas Pollard

Article

Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

mEOS3.2

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pFTK036

Plasmid

#171308

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

amdS ANIA_08777 acetamidase, selection marker

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pUM

Plasmid

#64176

Purpose

harbours marker genes URA3 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pAG425GAL-EGFP-ccdB

Plasmid

#14321

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pMEL10

Plasmid

#107916

Purpose

URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHS12-mCherry

Plasmid

#25444

Depositor

Benjamin Glick

Insert

mCherry

Use

Tags

COX4 Presequence

Expression

Yeast

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pMLB10

Plasmid

#177273

Purpose

GAL1p:T7RNAP(F11L;T613A):CYC1t

Depositor

Michael Jensen

Article

Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

Insert

T7RNAP(F11L;T613A)

Use

Tags

Expression

Bacterial and Yeast

Mutation

F11L T613A

Promoter

GAL1

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pHRW32

Plasmid

#130972

Purpose

HyPr plasmid with ble marker

Depositor

Chris Todd Hittinger

Article

Peris et al Nat Commun. 2020 Apr 29;11(1):2085. doi:

Insert

empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-mMaple3-kanMX6

Plasmid

#105165

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

Insert

mMaple3-kanMX6

Use

Tags

GlySerGly-mMaple3

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRS303 Gal TDP-43 YFP

Plasmid

#27468

Depositor

Aaron Gitler

Article

Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

Insert

TDP-43 (TARDBP Human)

Use

Tags

YFP

Expression

Yeast

Mutation

D23A mutation in TDP-43 sequence

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only