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Plasmid#181711PurposeGalactose inducible expression of FUSDepositorInsertFUS (FUS Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETcon(-)
Plasmid#41522DepositorTypeEmpty backboneUseTags3x(G4S) Linker, G3S Linker, HA, Myc Tag, and Stuf…ExpressionYeastMutationPromoterGAL1Available sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
YIP sHRPa-Aga1P
Plasmid#73151PurposeLarge fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.DepositorInsertsHRPa-Aga1P
UseTagsV5 tagExpressionYeastMutationPromoterglyceraldehyde-3-phosphate dehydrogenase GPD prom…Available sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
FRP880_PACT1(-1-520)-LexA-ER-haB112-TCYC1
Plasmid#58437PurposeExpresses LexA-ER-B112 in yeast cellsDepositorInsertLexA-ER-B112
UseTagsExpressionYeastMutationPromoterAct1 promoter (-520;1)Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJW1662
Plasmid#112050PurposeC-terminal tag with - AID*(AtIAA17_71-114)-HA and OsTIR1 expression cassette marked with LEUDepositorInsertsIAA17
TIR1
UseTagsHAExpressionYeastMutationonly amino acids 71-114PromoterADH1 and NoneAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSPObooster
Plasmid#216160PurposePlasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strainsDepositorUseTagsExpressionYeastMutationMKT1 gene containing an aspartic acid to glycine …Promoterendogenous promoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM184-ParB2::eGFP
Plasmid#87252PurposeExpresses ParB2-EGFP in yeast. Also known as pOR2-GFPDepositorInsertParB2
UseTagsEGFPExpressionYeastMutationPromoterpBAD/tetOAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYDS2.0_AT118-H
Plasmid#222846PurposeYeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)DepositorInsertAT118-H
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 mStayGold-T2A-mCherry
Plasmid#219834Purposeequimolar expression of mStaygold and mCherry in budding yeastDepositorInsertmStayGold-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-sfGFP-kanMX (pMaM17)
Plasmid#173452PurposeContains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP-ADH1ter
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA (V5 and HA)
Plasmid#222485PurposeCo-expression of two genes in yeast with V5 and HA tags and URA3 selectionDepositorTypeEmpty backboneUseTagsHA and V5ExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAR-Ec633
Plasmid#130873PurposeA nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRepDepositorInsertTP-DNAP1
UseTagsExpressionYeastMutationL477V, L640Y, I777K, W814NPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHCA/GAL4(1-93).ER.VP16
Plasmid#108216PurposeYeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domainDepositorInsertGal4 DBD-ERalpha HBD-VP16
UseTagsExpressionYeastMutationERalpha HBD has G400V mutationPromoterADH1Available sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mNeonGreen-HIS3MX6
Plasmid#129100PurposeFor tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreenDepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-Hook3
Plasmid#198525PurposeExpression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertHook3 (HOOK3 Human)
UseTagsGAL4-DNA binding domain (BD) fragmentExpressionYeastMutationPromoterADH1Available sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
BB3cK_pGAP_23*_pTEF_Cas9
Plasmid#104909PurposehCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selectionDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRCC-K
Plasmid#81191PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYZ463
Plasmid#187971PurposeTEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4tDepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsSV40ExpressionYeastMutationPromoterTEF1Available sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUDP004
Plasmid#101165PurposeE. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYES2-HTH
Plasmid#86470PurposeYeast expression vector containing HIS10-TEV-HA (HTH) epitope tag downstream of the GAL1 promoterDepositorTypeEmpty backboneUseTagsHIS10-TEV-HAExpressionYeastMutationPromoterGAL1Available sinceJan. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits