pHCA/GAL4(1-93).ER.VP16
(Plasmid #108216)

• Purpose: Yeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domain
• Depositing Lab: Didier Picard
• Publication: Louvion et al Gene 1993 Sep 6;131(1):129-34.

Backbone

• Vector backbone: pHCA
• Backbone size w/o insert (bp): 6408
• Vector type: Yeast Expression
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gal4 DBD-ERalpha HBD-VP16
• Mutation: ERalpha HBD has G400V mutation
• Promoter: ADH1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry