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Showing: 1101 - 1120 of 7341 results

  2. pFA NuiHA-loxP-nat-CUP1p

    Plasmid
    #131170
    Purpose
    PCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to Nourseothricin
    Depositor
    Nils Johnsson
    Article
    Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:
    Type
    Empty backbone
    Use
    Tags
    Nui-HA
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131170-plasmid-map-sequence-id-255831

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  4. pFA6a-hphMX-(3×FLAG)-TEV-ProtA

    Plasmid
    #52692
    Purpose
    For C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtA
    Depositor
    Michael Nick Boddy
    Insert
    (3×FLAG)-TEV-ProtA
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    n/a
    Available since
    April 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    52692-plasmid-map-sequence-id-82002

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  6. pACT2-CAS9c

    Plasmid
    #51055
    Purpose
    Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.
    Depositor
    Yunde Zhao
    Article
    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1
    Insert
    Cas9c
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    yeast ADH1 promoter
    Available since
    Feb. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51055-plasmid-map-sequence-id-77327

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  10. pJO629

    Plasmid
    #104342
    Purpose
    Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
    Depositor
    Alexander Varshavsky
    Article
    Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2
    Insert
    pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
    Use
    Tags
    3xFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    104342-plasmid-map-sequence-id-207903

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  17. pYZ960

    Plasmid
    #187972
    Purpose
    TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3
    Depositor
    Jef Boeke
    Article
    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.
    Insert
    Cas9 (codon optimized for human)
    Use
    Synthetic Biology
    Tags
    SV40
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    187972-plasmid-map-sequence-id-409168

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Showing: 1101 - 1120 of 7341 results