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pI1-MTU-DO-G418

Plasmid

#160012

Purpose

Integrative vector for K. marxianus, targeting the LAC4 locus. G418 selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Gal:mCherry-uTEV3

Plasmid

#135453

Purpose

Yeast expression of uTEV3 fused to mCherry

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

mCherry-uTEv3

Use

Tags

mCherry

Expression

Yeast

Mutation

S219V mutation improves stability , I138T/S153N/T…

Promoter

Gal

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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p415-PtrpC-Cas9-TtrpC-CYC1t

Plasmid

#68059

Purpose

Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

Depositor

Christian Hong

Article

Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

Insert

humanized Cas9

Use

CRISPR; N. crassa, fungi

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCYC1m_yeGFP

Plasmid

#64389

Purpose

encodes yeast enhanced GFP under control of minimal pCYC1 promoter

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

pCYC1

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pI3-MTU-DO-G418

Plasmid

#160023

Purpose

Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). G418 selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pESC-URA-GFP-Ubc9ts

Plasmid

#20369

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Mutation

Y68L This mutation renders Ubc9 temperature-sens…

Promoter

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pKP112 (pRS406-GFP-ADH1t)

Plasmid

#106472

Purpose

Vector for creating C-terminal GFP fusion proteins in yeast.

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Type

Empty backbone

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

None

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pIYC04

Plasmid

#64741

Purpose

Yeast expression plasmid

Depositor

Jens Nielsen

Article

Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pXP420

Plasmid

#26844

Depositor

Suzanne Sandmeyer, Nancy DaSilva

Article

Fang et al Yeast. 2010 Sep 10. ():.

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only

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p404ADH1

Plasmid

#15970

Depositor

Fred Cross, Nicolas Buchler

Insert

ADH1 promoter + polylinker + CYC1 terminator (ADH1 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

ADH1 promoter is between SacI and XbaI restrictio…

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pAW212

Plasmid

#172196

Purpose

P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites

Depositor

Chang Liu

Article

Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

Insert

p10B2-AT110

Use

Tags

Expression

Yeast

Mutation

Promoter

10B2 (P1 specific promoter)

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAG306GPD-ccdB-DsRed

Plasmid

#14356

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

DsRed

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pRG207

Plasmid

#64530

Purpose

Integrative E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pG2

Plasmid

#162603

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pUDP082

Plasmid

#103875

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

ScTDH3

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-ymNeongreen-CaURA3

Plasmid

#125703

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeongreen

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-tdTomato-hphMX6

Plasmid

#105157

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Zhu et al Mol Biol Cell. 2013 Oct;24(20):3187-204.

Insert

tdTomato-hphMX6

Use

Tags

tdTomato

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pTDH3-dCas9-Mxi1

Plasmid

#46921

Purpose

Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter

Depositor

Jonathan Weissman, Stanley Qi

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9-Mxi1

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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p413TEF-Sir2

Plasmid

#51742

Purpose

overexpression of Sir2 in S. cerevisiae, HIS3 marker

Depositor

Markus Ralser

Article

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

Sir2 (SIR2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCE41

Plasmid

#174433

Purpose

C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.

Depositor

Clarissa Nobile

Article

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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