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pSAH49 - [PVQp | mScarlet | tbb-2 UTR]
Plasmid#200325PurposemScarlet BioPart for PVQ neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[PVQp | wrmscarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003755, nlp-17Available sinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pPD30_38
Plasmid#1443DepositorTypeEmpty backboneUseTagsunc54enh, unc545', IVSA, rf2128ExpressionWormMutationPromoterAvailable sinceMarch 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMLS328
Plasmid#73717PurposeC. elegans germline CRE expression vectorDepositorInsert2xNLS-CRE
UseCre/LoxTagsExpressionWormMutationPromoterPeft-3Available sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCCM935
Plasmid#58202PurposeExpresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.DepositorInsertunc-22 sgRNA
UseTagsExpressionWormMutationPromoterU6Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJH4260
Plasmid#191678PurposePtwk-40s-GCaMP::wScarlet unc-54 3' UTR C.elegans AVA/other neurons expression GCaMP6s wScarletDepositorInsertGCaMP6s
UseTagswScarletExpressionWormMutationPromoterPtwk-40Available sinceNov. 30, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMLS256
Plasmid#73715PurposeSapTrap destination vector for building combined sgRNA expression + repair template vectorsDepositorInsertpU6::sgRNA
UseCRISPRTagsExpressionWormMutationSapI insertion sitePromoterC. elegans U6 snRNA pol III promoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-Cas9(dpiRNA)
Plasmid#107940Purposeeft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.DepositorInsertCas9(dpiRNA)
UseTagsExpressionWormMutationPromotereft-3Available sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBCN41-R4R3
Plasmid#34916DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHIP_RNA2
Plasmid#61260PurposeOrsay virus RNA2 genome segment expression plasmid for virus reverse geneticsDepositorInsertOrsay RNA2
UseTagsExpressionWormMutationPromoterheat shock promoterAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHIP_RNA1
Plasmid#61259PurposeOrsay virus RNA1 genome segment expression plasmid for virus reverse geneticsDepositorInsertOrsay RNA1
UseTagsExpressionWormMutationPromoterheat shock promoterAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2
Plasmid#182359PurposePositive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.DepositorInsertUltra-pan-neuronal positive control pUNP::gfp(NLS)
UseTagsExpressionWormMutationPromoterPromoter Ultra Pan NeuronalAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGM48DEST
Plasmid#74757PurposeEncodes for the QS repressor gene, splice linker, and mCherry reporter geneDepositorInsertsQS
Gateway cassette
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLS286
Plasmid#73723PurposeSaptrap tagRFP + Cbr-unc-119 donor plasmidDepositorInserttagRFP + Cbr-unc-119
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBN280
Plasmid#86731PurposeTissue-specific FLP expression plasmid for C. elegansDepositorInsertPtph-1::FLP D5
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
punc-119cR
Plasmid#21596DepositorInsertunc-119:mCherry (unc-119 Nematode)
UseAdds unc-119 marker to amp resistant plasmidsTagsmCherryExpressionWormMutationN/APromoterAvailable sinceAug. 26, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCZGY2851
Plasmid#87761PurposePunc-17b-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in cholinergic motor neurons.DepositorInsertPunc-17b-PH-miniSOG(Q103L)
UseTagsPHExpressionWormMutationPromoterPunc-17bAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUA97
Plasmid#69340PurposeIt encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategyDepositorInsertceBFP SEC 3xFlag
UseTags3xFlag and ceBFP (mTagBFP)ExpressionWormMutationsqt-1(e1350)PromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pmec-18 cz::ced-3 (p17) [TU#807]
Plasmid#16081DepositorInsertcz:: ced-3 (p17) (ced-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2008AvailabilityAcademic Institutions and Nonprofits only
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