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pDGE3

Plasmid

#79442

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE64

Plasmid

#79446

Purpose

recipient plasmid [single nuclease]; pPcUbi:Cas9, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE1

Plasmid

#79440

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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tRNA-gRNA position [D1_2] (GB1205)

Plasmid

#75406

Purpose

tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pTaCas9_5

Plasmid

#91168

Purpose

Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAtCas9-H840A_2

Plasmid

#91163

Purpose

Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_H840A (nickase)

Use

CRISPR

Tags

Expression

Plant

Mutation

H840A

Promoter

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pZA27

Plasmid

#158505

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-4xMyc. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-4xMyc (ZAR1 Mustard Weed)

Use

Tags

4xMyc

Expression

Plant

Mutation

L17E

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ291

Plasmid

#129671

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

BthCas12b

Use

CRISPR; Gateway compatible bthcas12b entry clone

Tags

Expression

Plant

Mutation

BthCas12b is rice codon optimized

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pUBQ10::ZF1CACTA1_3xFLAG_TET1cd

Plasmid

#106434

Purpose

pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsis

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

TET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)

Use

Synthetic Biology

Tags

3xFLAG

Expression

Plant

Mutation

Promoter

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDGB1_alpha1R

Plasmid

#68226

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDGB1_alpha2R

Plasmid

#68227

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE673

Plasmid

#153239

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)

Plasmid

#160647

Purpose

module containing the human codon optimized Cas9 and the BASTA selection marker

Depositor

Diego Orzaez

Insert

BASTA / Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos, P35S

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 TUB9p

Plasmid

#153373

Purpose

Plant promoter

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

TUB9 promoter

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pWRKY70::LUC

Plasmid

#141171

Purpose

Luminescent reporter for salicylic acid signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtWRKY70 promoter

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

AtWRKY70, AT3G56400

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pEGB3_alpha1 Pnos:P19:Tnos (GB2762)

Plasmid

#160632

Purpose

TU for expression of P19 driven by Nos promoter.

Depositor

Diego Orzaez

Insert

P19

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL11057

Plasmid

#68253

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_1 in barley

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promote:TaU6+sgRNA HvPM19_1

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pFRETvr-2in1-NN

Plasmid

#105126

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Use

Tags

mVenus/tagRFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTKan-p35S::Csy4-pNOS::cogRFP (C134, JBEI-15901)

Plasmid

#110146

Purpose

Transformation and expression of Csy4 and cogDsRed proteins in plants

Depositor

Dominique Loque

Article

Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

Insert

Csy4 (ATCS Mustard Weed)

Use

Tags

cogRFP

Expression

Bacterial and Plant

Mutation

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM865-5p

Plasmid

#84186

Purpose

target miRNA865-5p for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM865-5p

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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