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Plasmid#91108PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: noneDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only
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HBT_pcoCASphi_version2
Plasmid#197932PurposeCloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminalDepositorInsertpcoCasphi-2
UseCRISPRTags2xNLS-2xFLAGExpressionPlantMutationPromoter35SPPDK promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
BM00654
Plasmid#155339PurposeBinary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC64422
Plasmid#191768PurposeCRISPR mutagenesis in Medicago truncatulaDepositorInsertSpCas9
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
gRNA NIA TLS1
Plasmid#196980PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.DepositorInsertAtNIA1 gRNAs fused to TLS1 tags
UseTagsExpressionPlantMutationPromoterpU6-26, pU6-29Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 omega1 P35S:MS2:VPR:Tnos - P35S:dCas9:EDLL:Tnos (GB2085)
Plasmid#160645PurposeModule for the expression of Ms2 protein fused to VPR and dCas9 fused to EDLLDepositorInsertP35s-Ms2:VPR-Tnos-35s-dCas9:EDLL-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationBsmBI sites removedPromoter35S x2Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB508-cTP-sfGFP
Plasmid#213863PurposeBinary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)DepositorInsertPlastid transit peptide fused sfGFP
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRMTK-clo10
Plasmid#203961PurposeCloning plasmid containing the erythromycin resistance gene insertDepositorInsertErythromycin Resistance Gene
UseTagsExpressionPlantMutationPromoterN/AAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
35S::GeNL/pCAMBIA1301
Plasmid#190070PurposeExpressing bioluminescent pH indicator-GeNL- in Arabidopsis thalianaDepositorInsertGreen-enhanced Nano-lantern
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
35S::Cel1SP-GeNL/pCAMBIA1301
Plasmid#190069PurposeExpressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplastDepositorInsertCel1 signal peptide-Green-enhanced Nano-lantern
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB504
Plasmid#74846PurposeGateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterNo PromoterAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe
Plasmid#183161PurposeConstruct for transient expression of cell nucleus-, cytoplasm-targeted mCherry in plants.DepositorInsertmCherry
UseTagsExpressionPlantMutationPromoterAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCBL101.1
Plasmid#199724PurposeA pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene. BsaI site was removed.DepositorInsertMultiple Cloning Site, NPTII
UseTagsExpressionPlantMutation"GAGACC" to "GATACC"PromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35S:YFP:Tnos (GB0209)
Plasmid#68219PurposeTranscriptional unit for Yellow Flurescent Protein plant expression driven by the 35S promoterDepositorInsertYFP
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGWB541
Plasmid#74875PurposeGateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
lvl0 pOp6-35S
Plasmid#153409PurposeDex system inducible promoterDepositorInsertpOp6-35S promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRGEB32-BAR
Plasmid#126072PurposeFor CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoter35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…Available sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGWB413
Plasmid#74807PurposeGateway cloning compatible binary vector for C-terminal fusion with 3xHA (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
YFC43
Plasmid#89488Purposegateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing C terminal fragment of YFPDepositorTypeEmpty backboneUseTagsYFP-C (aa 154-240)ExpressionPlantMutationPromoter2x35SAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGE010
Plasmid#71536PurposeFor CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only