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Plasmid#177683PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editingDepositorInsertFokI-dMb2Cas12a
UseCRISPR; Gateway compatible foki-dmb2cas12a entry …TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
lvl0 AlcR
Plasmid#153410PurposeEthanol inducible system trans activatorDepositorInsertAlcR CDS
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSiM24-eGFP
Plasmid#67883PurposePlant Gene expression Vector pSIM24 containing eGFP reporterDepositorInserteGFP
UseTagsExpressionPlantMutationPromoterM24Available sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
gRNA NIA TLS2
Plasmid#196981PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.DepositorInsertAtNIA1 gRNAs fused to TLS2 tags
UseTagsExpressionPlantMutationPromoterpU6-26, pU6-29Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSW648 - pREV>>
Plasmid#115996Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpREV:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRm43GW
Plasmid#133748PurposeThree-fragment Multisite Gateway vector construction for red fluorescent seed selection in Arabidopsis thalianaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAXY0015
Plasmid#205584PurposeNpuDnaE(C)_HYG(90-341)_RUBYDepositorInsertNpuDnaE(C)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0014
Plasmid#205583PurposeHYG(1-89)-NpuDnaE(N)_GFPuvDepositorInsertNpuDnaE(N)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB115
Plasmid#68569PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged Citrine (Citri…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMM112
Plasmid#127229Purpose35S::IPT::pinII terminatorDepositorInsertIPT
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
ePPEplus
Plasmid#205241PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertc-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
L2_lacZgRNA-Cas9-CsA
Plasmid#136138PurposePlasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.DepositorInsertp5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35S:DsRed:Tnos (GB0361)
Plasmid#68220PurposeTranscriptional unit for DsRed plant expression driven by the 35S promoterDepositorInsertDsRed
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
35S-GWY-VP16/pAM
Plasmid#201232PurposeUsed to produce and express in planta C-terminal fusion proteins with the VP16 activation domainDepositorInsert35S-GWY-VP16
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPPED
Plasmid#162468PurposeDestination Expression vector for dicot plant prime editingDepositorInsertsCas9H840A
attR gateway destination cassette
UseTagsMLV reverse transcriptaseExpressionPlantMutationChange histidine 840 to Alanine based sequence o…Promoter2x35SAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB404
Plasmid#74798PurposeGateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterNo PromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPY22-mNeonGreen-Hyg
Plasmid#137082PurposeCodon-optimized mNeonGreen for expression in Arabidopsis thaliana and Physcomitrella patensDepositorInsertmNeonGreen-Flag
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJY-RpABE
Plasmid#112872Purposebinary vector for ABE expression in ArabidopsisDepositorInsertsRPS5A promoter
ABE7.10
U6-sgRNA cassette
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ142-ZmUbi-tRNA
Plasmid#158578PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-AtUBQ10:HR1b-GFP-T35S
Plasmid#211837PurposePlant transient expression cassette for HR1b-GFP fusion under UBQ10 promoter.DepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationPromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only