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PB-GFP-Gal8

Plasmid

#127191

Purpose

Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.

Depositor

Jordan Green

Article

Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

Insert

GFP-Gal8 (LGALS8 Human)

Use

Tags

Gal8 is fused to the c-terminus of GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-mCh-Cry2WT

Plasmid

#101221

Purpose

Blue-light dependent optogenetic protein Cry2

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

Cry2PHR

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Kv7.1/pcDNA3.1

Plasmid

#111452

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 1 isoform 1 [Homo sapiens] (KCNQ1 Human)

Use

Tags

8xHis and EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-JEDI-2P-WPRE

Plasmid

#179464

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Has Service

AAV9

Insert

JEDI-2P

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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BFP-KDEL

Plasmid

#49150

Purpose

visualization of the ER

Depositor

Gia Voeltz

Article

Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

Insert

BiP ER signal sequence (HSPA5 Human)

Use

Tags

BFP and KDEL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO (pEHA1339)

Plasmid

#209088

Purpose

Backbone plasmid for Tet Responsive transgene expression, FRT integration

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CalR-SNAP-tag2-KDEL

Plasmid

#226517

Purpose

Mamalian cell expression of SNAP-tag2 in endoplasmic reticulum

Depositor

Kai Johnsson

Article

Kühn et al bioRxiv 2024.08.28.610127

Insert

CalR-SNAP-tag2-KDEL

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP10

Plasmid

#22543

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP10 (USP10 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

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LentiGuide-puro-NLS-mCherry

Plasmid

#185474

Purpose

Mammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Type

Empty backbone

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3 BRE Luciferase

Plasmid

#45126

Depositor

Peter ten Dijke, Martine Roussel

Article

Korchynskyi et al J Biol Chem. 2002 Feb 15;277(7):4883-91.

Insert

Id1-BRE

Use

Luciferase; Bmp/smad transcriptional reporter

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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lenti U6-sgRNA/EF1a-mCherry

Plasmid

#114199

Purpose

Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

sgRNA scaffold

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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EGFR WT

Plasmid

#11011

Purpose

Retroviral construct for expressing human EGFR in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau E14

Plasmid

#46907

Purpose

expresses EGFP tagged Tau E14 in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau E14 (MAPT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

all 14 S/P or T/P amino acid residues (T111, T153…

Promoter

CMV

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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SF-SON IRES Puro

Plasmid

#214402

Purpose

Expresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)

Depositor

Tanja Kuhlmann

Article

Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-SRRM2-mCherry

Plasmid

#174089

Purpose

Inducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on system

Depositor

Katie Ullman, Wesley Sundquist

Article

Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

Insert

SRRM2 (SRRM2 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

TREtight

Available since

Sept. 12, 2021

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER Long P2A

Plasmid

#164107

Purpose

Detect the long Mitochondria-Endoplasmic reticulum contact

Depositor

Tito Calì, Marisa Brini

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Mt-ER Long P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.HI.eGFP-Cre.WPRE.SV40

Plasmid

#105545

Purpose

AAV expression of EGFP-Cre from CMV promoter

Depositor

James M. Wilson

Has Service

AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

EGFP-Cre

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

HI

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag mTOR wt

Plasmid

#26603

Purpose

Mammalian expression of human flag-tagged mTor

Depositor

Jie Chen

Article

Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

Insert

mTOR (MTOR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only

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E326K GBA pcDNA3.1

Plasmid

#188581

Purpose

Ectopic expression E326K GBA

Depositor

Anthony Schapira

Article

Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

Insert

GBA (GBA1 Human)

Use

Tags

Expression

Mammalian

Mutation

E326K

Promoter

CMV

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pENN239_CRISPRcharm_Kv1

Plasmid

#220838

Purpose

CRISPRcharm Kv1 expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

CRISPRcharm Kv1

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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