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Plasmid#140022PurposePlasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integrationDepositorInsertPcKRS (pylS Methanosarcina bakeri)
UseTagsFLAGExpressionMammalianMutationM241F, A267S, Y271C, L274MPromoterEF1Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO eGFP Puro (w159-1)
Plasmid#17481Purpose3rd gen lentiviral eGFP expression vector, CMV/TO promoter, PuroÂDepositorInsertenhanced green fluorescent protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-c/o NL4-3 Env delta CT
Plasmid#139285PurposeHIV-1 NL4-3 glycoprotein lacking cytoplasmic tailDepositorInsertcodon optimized HIV-1 Env delta cytoplasmic tail
UseTagsExpressionMammalianMutationdeletion of cytoplasmic tail after residue 710PromoterCMV IEAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_APEX_FLAG_Nt
Plasmid#194068Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsV5-APEX-FLAGExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING-342STOP
Plasmid#102588PurposeLentivector to express STING delta 342 and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationdelta 342PromoterSFFVAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mTagBFP2
Plasmid#175509PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF3229
Plasmid#144705PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMAX (MAX Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1261
Plasmid#143779PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1262
Plasmid#142772PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1260
Plasmid#142771PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1259
Plasmid#142770PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1258
Plasmid#142769PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1257
Plasmid#142768PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1256
Plasmid#142767PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1255
Plasmid#142766PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1254
Plasmid#142765PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0047
Plasmid#141492PurposeLentiviral vector for overexpressing the MAX transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMAX (MAX Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Td-CBEmax
Plasmid#196600PurposeMammalian expression of Td-CBEmaxDepositorInsertbpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS
UseTags6*HisExpressionMammalianMutationTadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)PromoterAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Halo-Cyto
Plasmid#124314PurposeExpresses cytosolic specific HaloTag proteinDepositorInsertHalo tag
UseTagsCytoplasm specific seqExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC52A1
Plasmid#132169PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC52A1 (SLC52A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits