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pCMV-T7-SpCas9-P2A-EGFP (RTW3027)
Plasmid#139987PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SlugABE-NNG
Plasmid#214356PurposeExpresses SlugABE-NNGDepositorInsertSlugABE-NNG
UseAAVTagsExpressionMammalianMutationQ782R/S888R/L906R/N984S/E1012K/K1016IPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFSF13-Fc(DAPA)-AviTag-6xHis
Plasmid#156611PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertTNFSF13 (TNFSF13 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7
Plasmid#175526PurposeCo-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cellsDepositorInsertLifeact-HaloTag11 and Tomm20-HaloTag7
UseTagsExpressionMammalianMutationHaloTag11 = HaloTag7-M175WPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-LIG3
Plasmid#215143PurposeExpresses mNeonGreen-LIG3 in mammalian cells from a retroviral vector.DepositorInsertLIG3 (LIG3 Human)
UseRetroviralTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pSicoR p53
Plasmid#12090DepositorInsertp53 shRNA (Trp53 Mouse)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationEGFP is expressed from this plasmid as a marker, …PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Ran-Q69L-mRFP1-polyA
Plasmid#104560PurposeExpress Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertRan (Ran Mouse)
UseTagsmRFP1ExpressionMammalianMutationQ69LPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1_WiChR_TS_mScarlet_ER
Plasmid#195190PurposeHigh efficient, K+ selective channelrhodopsin for the inhibition of excitable cellsDepositorInsertWobblia lunata inhibitory Channelrhodopsin
UseTagsER-export sequence, Kir2.1 trafficking motif, and…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
CRY2-OCRL
Plasmid#79566Purposeexpression of CRY2PHR fused to inositol 5-phosphatase domain of OCRLDepositorInsertOCRL (OCRL Human)
UseTagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationaa 234_539PromoterCMVAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-Rab7(WT)
Plasmid#112959PurposeIn vivo visualization of Rab7 (can be used for colocalization studies)DepositorArticleInsertRab7
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-VARNAM2-Kv2.1PR
Plasmid#195530PurposeRed fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targetedDepositorInsertVARNAM2-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationVARNAM SI linkerPromoterCaMKIIAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-ABEmax-BlastR
Plasmid#152989PurposeA-to-G base editorDepositorInsertABE7.10-nSpCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro-SOX2
Plasmid#47540PurposeTet inducible knockdown of SOX2.DepositorInsertSOX2 shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP
Plasmid#160697PurposeExpresses GFP in mammalian cellsDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase
Plasmid#119816Purpose3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cellsDepositorInsertsLuciferase
Puromycin
UseLentiviral and LuciferaseTagsEGFP and V5ExpressionMammalianMutationStop codon removed in the frame of the V5 frame v…PromoterCMV/TO and Human Phosphoglycerate kinaseAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5 TGF beta type I receptor Flag
Plasmid#14831DepositorInsertTGF beta type I receptor (Tgfbr1 Rat)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
SuExp His-Myc-mPLD4
Plasmid#201185PurposeProtein expression plasmid for recombinant His-Myc tag mouse PLD4DepositorUseTagsHis-Myc, Factor Xa cleavableExpressionMammalianMutationcytosolic and transmembrane domain truncated; add…PromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
iRFP-C1
Plasmid#54786PurposeLocalization: C1 Cloning Vector, Excitation: 690, Emission: 713DepositorTypeEmpty backboneUseTagsiRFPExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits
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