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Plasmid#182147PurposeTo insert attB attachment site at CCR5 in human cells via twinPEDepositorInsertCCR5-A509b-attB pegRNA
UseTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe B-Raf V600E
Plasmid#17544DepositorInsertB-Raf (BRAF Human)
UseRetroviralTagsExpressionMammalianMutationV600EPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Flag CASTOR1 I280A
Plasmid#84495Purposeoverexpression of CASTOR1 I280A mutantDepositorInsertCASTOR1 I280A (GATSL3 Human)
UseTagsFLAGExpressionMammalianMutationI280APromoterCMVAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
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MAC_C_COX41
Plasmid#111664PurposeMAC-tagged gene expressionDepositorInsertCOX41 (COX4I1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-Sestrin2-E451A
Plasmid#73673PurposeoverexpressionDepositorInsertSesn2 (SESN2 Human)
UseTagsFLAGExpressionMammalianMutationE451A changed Glutamate 451 to AlaninePromoterCMVAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato
Plasmid#180152PurposetdTomato cre reporter with Rosa26 recombineering homology armsDepositorInserttdTomato
UseCre/Lox and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
UBC plasmid
Plasmid#169746PurposeUnregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterUBCAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Peroxisomes-2
Plasmid#54520PurposeLocalization: Peroxisomes, Excitation: 587, Emission: 610DepositorTypeEmpty backboneUseTagsPeroximal Targeting Signal 1 (PTS1) and mCherryExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-hDF-C2A
Plasmid#72582Purposehuman dysferlin C2A domain tagged with YFPDepositorInsertdysferlin C2A domain (DYSF Human)
UseTagsEYFPExpressionMammalianMutationC2A domainPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-tTA
Plasmid#104102PurposeExpresses tTA2 under the CAG promoter in mammalian cells.DepositorInserttTA2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
BII-CMV-AfmW3A
Plasmid#133402PurposePiggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cellsDepositorUseTagsExpressionMammalianMutationN/APromoterCMVAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-Zeo-BsmBI_entry (pRW1500)
Plasmid#225753PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Zeocin resistance; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human USP7-3X FLAG
Plasmid#225334PurposeLentiviral expression of human USP7 with 3x FLAG tag in mammalian cellsDepositorInsertUSP7 (USP7 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE pGK hNLRP3-mNG
Plasmid#218643PurposeExpresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak pGK promoter with blast selectionDepositorInsertNLRP3 (NLRP3 Human)
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterpGKAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2091
Plasmid#144382PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFANCD2 (FANCD2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-StrepII
Plasmid#208617PurposeEmpty vector for mammalian expression, insert a Strep II tag (with ATG) using XbaI and XhoI site of pcDNA3.1 (-), both site retainedDepositorTypeEmpty backboneUseTagsStrep IIExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0058
Plasmid#143007PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMAF (MAF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2902
Plasmid#143658PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertDACH1 (DACH1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mVenus1-155-GGGS-TDP43 M337V
Plasmid#162612PurposeAllows for transcription of mVenus fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assaysDepositorInsertTARDBP (TARDBP Human)
UseTagsExpressionMammalianMutationM337VPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only