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Plasmid#115145PurposeSafe harbor site 231 knock-in vector with RFP-HygromycinR expression cassetteDepositorInsertRFP
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
MscL(G22S)-c-term-cpGFP-ERexp
Plasmid#195346PurposeControl plasmid for the MscL tension biosensorDepositorInsertMscL(G22S)-c-term-cpGFP-ERexp
UseLentiviralTagsExpressionMammalianMutationPromoterCMVd2Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3450
Plasmid#144926PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertELK1 (ELK1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mito-B
Plasmid#209864PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVSDepositorInsertddGFP B
UseTagsTargeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5 CASTOR1-Flag Cterm
Plasmid#84488Purposeoverexpression of CASTOR1 with Flag tagDepositorInsertCASTOR1 (GATSL3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-eGFP-attP(bxb)-*BsdR
Plasmid#183751PurposeBxb1 landing pad cassette.DepositorInsertloxP-eGFP-attP-Bsd-lox251
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF1307
Plasmid#142371PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertJARID2 (JARID2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Flag-METTL3
Plasmid#53739PurposeFor expression of METTL3 (methyltransferase-like 3) in human cellsDepositorInsertMethyltransferase-like 3 (METTL3 Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterT7Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSaGuide
Plasmid#64710PurposeCloning and expression of Staphylococcus aureus guide RNADepositorInsertStaphylococcus aureus guide RNA
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-U6
Plasmid#19799DepositorTypeEmpty backboneUseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FLAG
Plasmid#183511PurposeGateway converted, destination vector with N-terminal Flag tag. For mammalian expression.DepositorTypeEmpty backboneUseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHis6-hDF-deltaTM-pCMV-9
Plasmid#72587Purposehuman dysferlin no transmembrane domain tagged with his tagDepositorInsertdysferlin (DYSF Human)
UseTagsHisExpressionMammalianMutationtransmembrane domain deletedPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-SIRT6-FLAG
Plasmid#102322PurposeHuman SIRT6 with FLAG tag for mammalian expressionDepositorInsertSIRT6 (SIRT6 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
hfDECOR-F148A
Plasmid#219546PurposeExpression of CMV-TadA8e-F148A-NLS-dCas13d-NLS in mammalian cells (hfDECOR-F148A expression)DepositorInserthfDECOR-F148A
UseCRISPRTagsExpressionMammalianMutationChanged TadA8e Phenylalanine 148 to AlaninePromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
DECOR
Plasmid#219545PurposeExpression of CMV-TadA8e-NLS-dCas13d-NLS in mammalian cells (DECOR expression)DepositorInsertDECOR
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
hfDECOR-V106G
Plasmid#219547PurposeExpression of CMV-TadA8e-V106G-NLS-dCas13d-NLS in mammalian cells (hfDECOR-V106G expression)DepositorInserthfDECOR-V106G
UseCRISPRTagsExpressionMammalianMutationChanged TadA8e Valine 106 to GlycinePromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Rac1-2G
Plasmid#66111PurposeSecond generation Rac1 FRET biosensor for lentivirus productionDepositorInsertSecond generation Rac1 FRET biosensor
UseLentiviralTagsHis-MycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
GZnP3 in pcDNA3.1+
Plasmid#161738PurposeCytosolic fluorescent reporter for Zn²⁺ (Kd = 1.3 nM, ex/em = 488 nm/515 nm)DepositorInsertGZnP3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
c-myc-PT3EF1a
Plasmid#92046PurposeExpresses c-Myc in mammalian cellsDepositorInsertC-MYC (MYC Human)
UseTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceJuly 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJR85
Plasmid#140095PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only