-
Plasmid#55052PurposeLocalization: Golgi Complex, Excitation: 587, Emission: 610DepositorInsertGolgi (B4GALT1 Human)
UseTagsmCherryExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE
Plasmid#179459PurposeDouble floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed under the mammalian promoter (EF1a)DepositorHas ServiceAAV1InsertJEDI-2P-Kv
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-Mito4x-jRCaMP1b
Plasmid#127873PurposeRed-shifted fluorescent reporter for mitochondrial matrix Ca2+DepositorInsertRed-shifted mitochondrial matrix Ca2+ indicator
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
SARS CoV-2 RBD (bio-His)
Plasmid#166857PurposeHuman expression vector encoding SARS CoV-2 receptor binding domain (RBD) (amino acids 319-541), containing biotinylation site and 6His tag.DepositorInsertSARS CoV-2 receptor binding domain (RBD) (S Severe acute respiratory syndrome-related coronavirus)
UseTags6xHis and Biotinylation siteExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
DRD1-Tango
Plasmid#66268PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertDRD1 (DRD1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_CalR_HaloTag9_KDEL
Plasmid#175540PurposeExpression of HaloTag9 in the endoplasmid reticulum of mammalian cellsDepositorInsertCalR-HaloTag9-KDEL
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_TNF-SBP-EGFP
Plasmid#65280Purposesynchronize trafficking of TNF from the ER (RUSH system)DepositorInsertTNF (TNF Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP11-pDEST-C
Plasmid#118367PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsGFP11-FLAGExpressionMammalianMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_HaloTag9_LamB1
Plasmid#175538PurposeExpression of HaloTag9 at the nuclear lamina of the mammalian cellsDepositorInsertHaloTag9-LamB1
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQHA-USP7 WT puroR
Plasmid#46753PurposeRetroviral vector that expresses wild type HA-tagged human USP7DepositorInsertUSP7 (USP7 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJR85
Plasmid#140095PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_PML_RARA
Plasmid#205897PurposeExpress mEGFP-tagged fusion protein, PML_RARA from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C-p50
Plasmid#180238PurposeExpresses p50 in mammalian cellsDepositorInsertp50 (DCTN2 Human)
UseTagspmCherry-C1ExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL0341 (pTarget)
Plasmid#200906PurposeEncodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.DepositorInsertpTarget
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSyn.Cre.hGH
Plasmid#105555PurposeAAV expression of Cre from hSyn promoterDepositorHas ServiceAAV9InsertCre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhSynAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Tubulin K40R
Plasmid#105303PurposeTo study effects of acetylation-null tubulinDepositorInsertalpha tubulin (TUBA1B Human)
UseTagsEGFPExpressionMammalianMutationK40RPromoterAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMN-296
Plasmid#195454PurposeLentiviral Integratable mCherry Reporter for synZiFTR ZF3DepositorInsert8X ZF3 BS - ybTATA - mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
miR34a in pcDNA3.1
Plasmid#78125PurposeMammalian expression of miR34DepositorInsertmiR34 (MIR34A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV Luciferase PGK-hygro
Plasmid#18782DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLuciferase and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
UBC plasmid
Plasmid#169746PurposeUnregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterUBCAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only