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EYFP-hDF-deltaTM-pCMV-9

Plasmid

#72586

Purpose

human dysferlin no transmembrane domain tagged with YFP

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin (DYSF Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

transmembrane domain deleted

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RLUC POLIRES FLUC

Plasmid

#45642

Purpose

Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

Depositor

Nahum Sonenberg

Article

Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

Insert

RLUC - IRES - FLUC

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-UtrCH

Plasmid

#26737

Depositor

William Bement

Article

Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

Insert

Utrophin

Use

Reporter for f-actin

Tags

GFP

Expression

Mammalian

Mutation

Calponin homology domain of utrophin (aa# 1-261).…

Promoter

CMV IE94

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO.DEST.puro

Plasmid

#32682

Purpose

Lentiviral cDNA/shRNA gateway destination vectors

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2014

Availability

Academic Institutions and Nonprofits only

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TRPC1-FLAG

Plasmid

#24408

Depositor

Craig Montell

Article

Xu et al Cell. 1997 Jun 27. 89(7):1155-64.

Insert

TRPC1 (TRPC1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pIRES2-hPAC-FLAG

Plasmid

#129494

Purpose

Expresses human TMEM206 tagged at the C-terminus with FLAG

Depositor

Zhaozhu Qiu

Article

Yang et al Science. 2019 Apr 26;364(6438):395-399.

Insert

human PAC tagged with FLAG tag at the C-terminal end (PACC1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLXRN-EGFP

Plasmid

#99207

Purpose

EGFP expressing bicistronic retroviral vector

Depositor

Peter Pedersen

Insert

EGFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

A Kozak sequence was incorporated to enhance tran…

Promoter

LTR

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-Tet1-CD

Plasmid

#83341

Purpose

To introduce MS2 coat protein fused Tet1-CD

Depositor

Ronggui Hu

Article

Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

Insert

MS2-Tet1-CD

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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2HA-pDEST-C

Plasmid

#118374

Purpose

Gateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-JEDI-2P-Kv-WPRE

Plasmid

#179463

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Has Service

AAV9

Insert

JEDI-2P-Kv

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSEMS-Tom20-mEGFP-reHaloTagF

Plasmid

#200190

Purpose

Tom20 with reversible HaloTagF variant for fluorescent labeling

Depositor

Jacob Piehler

Article

Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

Insert

Tom20 (TOMM20 Human)

Use

Tags

mEGFP and reHaloTagF

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-CAG-hrGFP

Plasmid

#52344

Purpose

AAVS1 donor vector for constitutive gene expression

Depositor

Su-Chun Zhang

Article

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

hrGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

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InsRA-eGFP

Plasmid

#79795

Purpose

Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

InsRA (INSR Human)

Use

Tags

eGFP extracellular

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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ALKBH5-dCas9

Plasmid

#134783

Purpose

Expression fusion protein ALKBH5-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

ALKBH5 and inactive dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-PP7-2xmCherry-CAAX

Plasmid

#74925

Purpose

Expresses PP7-2xmCherry-CAAX

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

PP7-2xmCherry-CAAX

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE3g-mNeon-WPRE-pA

Plasmid

#192064

Purpose

mNeonGreen expression with TRE3g promoter

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

mNeonGreen

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE3g

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApoE4

Plasmid

#197148

Purpose

Expresses ApoE4 in Mammalian cell

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE4 (APOE Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP Cited2

Plasmid

#112246

Purpose

expression of EGFP-Cited2 in mammalian cells

Depositor

Simone Di Giovanni

Insert

Cited2 (CITED2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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PB-CAG-ChR2-YFP

Plasmid

#204345

Purpose

PiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.

Depositor

Ivo Lieberam

Article

Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

Insert

ChR2-YFP

Use

Tags

Yellow fluorescent protein

Expression

Mammalian

Mutation

C-terminus (aa315-737) is deleted - PMID: 1461559…

Promoter

CAG

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-DJ-N589X

Plasmid

#130878

Purpose

Encodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codon

Depositor

Prashant Mali

Article

Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

Insert

AAV-DJ cap N589X

Use

Tags

Expression

Mammalian

Mutation

N589X

Promoter

Unknown

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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