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Plasmid#142863PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertEZH1 (EZH1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXR004: CasRx pre-gRNA cloning backbone
Plasmid#109054PurposehU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-GFP-Rabphilin
Plasmid#170865PurposeMammalian expression of His-GFP-Rabphilin.DepositorInsertRabphilin-3a (RPH3A Bovine)
UseTags(His)6 and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SMVP-Cas9N
Plasmid#80930PurposeExpresses Cas9N in mammalian cells.DepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac-TetO-En1-t2a-Foxa2-hygro
Plasmid#176483PurposeTetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistanceDepositorUseTagsExpressionMammalianMutationPromoterTetOAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMK444 (EF1-OsTIR1(F74G) Puro)
Plasmid#214374PurposeEF1-OsTIR1(F74G) (PiggyBac)DepositorInsertEF1-OsTIR1(F74G)
UseTagsExpressionMammalianMutationPromoterEF1 promoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFRT/FLAG/HA-DEST PUM2
Plasmid#40292DepositorInsertPumilio homolog 2 (PUM2 Human)
UseTagsFLAG-HAExpressionMammalianMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pShuttle CMV RGECO-TnT
Plasmid#124643PurposeMyofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombination.DepositorInsertRGECO-TnT (TNNT2 Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF0653
Plasmid#141651PurposeLentiviral vector for overexpressing the NR1I3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR1I3 (NR1I3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DestFLAGHA_RBPMS
Plasmid#65743Purposeconstitutively expresses RBPMS in mammalian cellsDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLS-SV40-mP-EGFP
Plasmid#137724PurposeLentivirus based EGFP expression vectorDepositorInsertSV40 enhancer
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 ASXL1 (p.G646 Wfs *12) 3x FLAG
Plasmid#74245PurposeCancer-associated ASXL1 truncation mutant in mammalian expression vectorDepositorInsertASXL1 (ASXL1 Human)
UseTags3X FLAGExpressionMammalianMutationp.G646 Wfs *12 truncationPromoterCMVAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF3325
Plasmid#144801PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertDLX3 (DLX3 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-HsSmg7_H
Plasmid#146476PurposeMammalian Expression of HsSmg7DepositorInsertHsSmg7 (SMG7 Human)
UseTagsExpressionMammalianMutationone non silent mutation G2560A (V854I) and three …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRDA_526
Plasmid#184859PurposeLentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.DepositorInsertsgRNA cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti GW V5 Eco/Dam mse EMD
Plasmid#182675PurposeLentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Emerin. Used in DamID experiments to methylate DNA that interacts in the proximity of Emerin.DepositorInsertEmerin (Emd Mouse)
UseLentiviralTagsE. Coli Dam and V5ExpressionMammalianMutationPromoterHSPAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 TaGu N-1F
Plasmid#203762PurposeFor expression of N-flag tagged TaGu R2 protein in mammalian cellsDepositorInsertR2 retroelement protein
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
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SRE reporter vector_559
Plasmid#82686PurposeCloning vector containing serum response element (SRE) followed by an AscI restriction siteDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2016AvailabilityAcademic Institutions and Nonprofits only