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Plasmid#194432PurposeAPOBEC1 fused to YTHD422NDepositorInsertAPOBEC1-YTHD422N (APOBEC1 Human)
UseCRISPRTagsAPOBEC1-YTHD422N-HAExpressionMammalianMutationD422N mutation in the YTH domain to enhance m6A b…PromoterAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RIPK1
Plasmid#78842PurposeTo overexpress RIPK1 in Mammalian CellsDepositorInsertRIPK1 (RIPK1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFH2.32_LV-mCherry-MS2x8-BCS
Plasmid#205539PurposeExpress mCherry-MS2x8 with barcodeDepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCralbp-DsRed
Plasmid#11158DepositorInsertCralbp promoter (Rlbp1 Mouse)
UseTagsDsRed2ExpressionMammalianMutationPromoterAvailable sinceApril 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/hChR2(H134R)-mCherry
Plasmid#20938PurposeMammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activationDepositorInsertchannelrhodopsin-2
UseTagsExpressionMammalianMutationH134R mutation in ChR2PromoterCMVAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLX317-SNAI1
Plasmid#115446PurposeConstitutive lentiviral expressionDepositorInsertSNAI1 (SNAI1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
HDAC1 Flag
Plasmid#13820PurposeMammalian expression of human histone deacetylase 1 with flag tagDepositorInsertHDAC1 (HDAC1 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Ataxin3Q84
Plasmid#22123DepositorInsertAtaxin 3 (ATXN3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
CMV-G-GECO1.2
Plasmid#32446PurposeMammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)DepositorArticleInsertG-GECO1.2
UseTagsExpressionMammalianMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
PEA1-Puro
Plasmid#171991PurposeDelivers all prime editing nickase components in a single, puromycin selectable plasmidDepositorInsertCbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
UseTagsExpressionMammalianMutationPromoterCMV for Cas9, U6 for gRNAsAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEJS2601_AAV-Nme2-ABE8e-i1-U6-2xSapI
Plasmid#201683PurposeAll-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 sgRNA cassette. SapI enables cloning new spacers.DepositorInsertNme2-ABE8e-i1
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU1aAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Neo-PIP-NLS-mVenus
Plasmid#118619PurposeLentiviral expression of the cell cycle marker PIP-NLS-mVenusDepositorInsertPIP-NLS-mVenus
UseLentiviralTagsmVenusExpressionMammalianMutationPromoterhPGKAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mRuby2
Plasmid#40260PurposeRFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlapDepositorHas ServiceCloning Grade DNAInsertmRuby2
UseTagsHis6 and XpressExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-Nluc-2xC/D box
Plasmid#180504PurposeMammalian expression of Nluc-2xC/D boxDepositorInsertNluc-2xC/D box
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
HyPer7.2DAAO-NES
Plasmid#168301PurposeChemogenetic fluorescent H2O2 reporter targeted to the cytoplasmDepositorInsertHyPer7.2DAAO-NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac-TetO-En1-t2a-Foxa2-hygro
Plasmid#176483PurposeTetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistanceDepositorUseTagsExpressionMammalianMutationPromoterTetOAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
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AAV-hSyn-Ribo-jGCaMP8s
Plasmid#167572PurposePlease see Addgene item #169247 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s.DepositorInsertRibo-GCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_Giantin_C1
Plasmid#85050PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorInsertGiantin (GOLGB1 Human)
UseTagsExpressionMammalianMutationaa 3131-3259PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
psPax2-D64V-NC-mNeon
Plasmid#196509PurposemNeon fluorescent protein fused to nucleocapsid protein in psPAX2DepositorInsertmNeon
UseTagsmNeonExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only