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Plasmid#180142Purposeused to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periodsDepositorInsertHuman LRP6 (LRP6 Human)
UseTagsExpressionMammalianMutationPromoterSP6Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-P2A-APEX2-csChBP
Plasmid#108877PurposeExpression of APEX2 fused to a conditionally stable nanobody against mCherry (includes green cytoplasmic reporter)DepositorInsertEGFP-P2A-APEX2-csChBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF0612
Plasmid#143054PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRBPJ (RBPJ Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
DmTmem63
Plasmid#136598PurposeExpresses the mechanosensitive channel Drosophila melanogaster Tmem63 in mammalian cellsDepositorInsertDmTMEM63
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-CRISPRoffv2.1-IRES-BlastR
Plasmid#207180PurposeLentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a PromoterDepositorInsertCRISPRoffv2.1
UseCRISPR and LentiviralTagsHA and TagBFPExpressionMammalianMutationPromoterEF1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Baf60c
Plasmid#72691PurposeDoxycyline regulated expression of Baf60c in mammalian cellsDepositorInsertBrg 1 (SMARCD3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF1834
Plasmid#142863PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertEZH1 (EZH1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1
Plasmid#72266PurposeExpress gene of interest under EF-1 promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
His-GFP-Rabphilin
Plasmid#170865PurposeMammalian expression of His-GFP-Rabphilin.DepositorInsertRabphilin-3a (RPH3A Bovine)
UseTags(His)6 and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pShuttle CMV RGECO-TnT
Plasmid#124643PurposeMyofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombination.DepositorInsertRGECO-TnT (TNNT2 Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF0653
Plasmid#141651PurposeLentiviral vector for overexpressing the NR1I3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR1I3 (NR1I3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DestFLAGHA_RBPMS
Plasmid#65743Purposeconstitutively expresses RBPMS in mammalian cellsDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRT006.3 L1 dual-luciferase AI retrotransposition reporter
Plasmid#213031PurposeBi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integrationDepositorInsertTet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
UseLuciferaseTagsFirefly luciferase antisense intron (3' UTR)ExpressionMammalianMutationPromoterpTRE bidirectional tet-onAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR171-Tango
Plasmid#66337PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPR171 (GPR171 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA(MS2) cloning backbone
Plasmid#61424PurposesgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP II (pMIG II)
Plasmid#52107Purposea bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsIRES-EGFPExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
eGFP-hCOL1A1
Plasmid#140110PurposeExpresses fluorescently-tagged human Type I procollagen α1 chain with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 (COL1A1 Human)
UseTagseGFPExpressionMammalianMutationCOL1A1 exons 2-3 replaced by fluorescent tag, K20…PromoterCMVAvailable sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
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TFORF3325
Plasmid#144801PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertDLX3 (DLX3 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3376
Plasmid#144852PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTOX (TOX Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only