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Plasmid#59759Purposeencodes multiple copies of an epitope tag HADepositorInsertsmFP _HA
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 - hSRSF6
Plasmid#120419Purposeoverexpression of human SRSF6DepositorInsertSRSF6 (SRSF6 Human)
UseTagsExpressionMammalianMutationPromoterCMV immediate/earlyAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
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TFORF0596
Plasmid#143226PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPLAG1 (PLAG1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
p7129 pHAGE-P-CMVt-N-HA-GAW-HM13
Plasmid#100155PurposeExpresses HM13DepositorInsertNM_030789.3 (HM13 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
ER-mNeonGreen
Plasmid#137804PurposeVisualization of the Endoplasmic ReticulumDepositorArticleInsertKDEL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pANAP
Plasmid#48696Purposeallows co-translational incorporation of a fluorescent AA directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRSDepositorInserts8 copies of the amber suppressor tRNACUA-Leu
AnapRS
UseTagsExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMV and human H1 promoterAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
CCR2-DuET
Plasmid#213198PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCR2 (CCR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA GSK3 beta S9A pcDNA3
Plasmid#14754PurposeMammalian expression of human GSK3 beta constitutively active mutant with HA tagDepositorInsertGSK3 beta S9A (GSK3B Human)
UseTagsHAExpressionMammalianMutationS9A. Constitutively active mutant.PromoterAvailable sinceApril 12, 2007AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV.CAG.iGluSnFR3.v857.GPI
Plasmid#178335PurposeFluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPIDepositorInsertpAAV CAG iGluSnFR3 v857.GPI
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsGPI Anchor, IgK-chain, and Myc epi tagExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiGuideFE-Puro
Plasmid#170069PurposeExpresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoterDepositorInsertCas9 guide RNA scaffold with the F+E scaffold modification
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tom20-mCh-FKBP
Plasmid#162436Purposefor use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) systemDepositorInsertTom20-mCh-FKBP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF1027
Plasmid#141784PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRARA (RARA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-Mfn1-E209A
Plasmid#141155PurposeMammalian expression and labeling of mutant mitofusin 1 (E209A) deficient in GTP hydrolysisDepositorInsertMFN 1 (MFN1 Human)
UseTagsAcGFPExpressionMammalianMutationchanged Glutamic acid at position 209 to Alanine …PromoterCMVAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-N-mCherry
Plasmid#123215PurposeN-terminal mCherry tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway destinationTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-full length LIC1
Plasmid#74601Purposeexpresses LIC1 in mammalian cells; untagged but cells indicate expression with diffuse mCherryDepositorInsertfull length human dynein light intermediate chain 1 (DYNC1LI1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 HA C9ORF72 op
Plasmid#74156PurposeExpress HA tagged optimized C9ORF72 in mammalian cellsDepositorInsertC9ORF72 (C9orf72 Synthetic, Human)
UseTagsHAExpressionMammalianMutationoptimized sequencePromoterAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
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Lenti-eCas9
Plasmid#140237PurposeLentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffoldDepositorTypeEmpty backboneUseCRISPR and LentiviralTags3xFLAGExpressionMammalianMutationPromoterEF-1a coreAvailable sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-HsSmg6_H
Plasmid#146475PurposeMammalian Expression of HsSmg6DepositorInsertHsSmg6 (SMG6 Human)
UseTagsExpressionMammalianMutationtwo silent mutations T594C, A699G and two non sil…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only