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Plasmid#51469PurposeBiosensor for PtdIns4PDepositorInsertP4M-SidM(546-647)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-Rac1-2G
Plasmid#66110PurposeSecond generation Rac1 FRET biosensorDepositorInsertSecond generation Rac1 FRET biosensor
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF2013
Plasmid#142521PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGATAD2A (GATAD2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-U6-sgMettl3
Plasmid#196263PurposeExpresses a Mettl3-targeting sgRNA driven by the U6 promoter and Cre-recombinase driven by the PGK promoterDepositorInsertMettl3 sgRNA (Mettl3 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
mito-PAGFP
Plasmid#23348PurposeExpresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.DepositorInsertPAGFP
UseTagsmitoExpressionMammalianMutationPromoterAvailable sinceApril 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
peGFP-N1-SHB
Plasmid#139605PurposeExpresses eGFP-SHB fusion protein in mammalian cellsDepositorInsertSHB (SHB Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nrf1-Myc pcDNA3.1(+)-C-Myc
Plasmid#169125Purposeexpression of proteinDepositorInserthNrf1 (NFE2L1 Human)
UseTagsExpressionMammalianMutationwtPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
MCS-13X Linker-BioID2-HA
Plasmid#80899PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-flag-hKDM4A(H188A)-polyA
Plasmid#101052Purposein vitro transcription (T7 promoter)DepositorInsertKDM4A (KDM4A Human)
UseTagsFlagExpressionMammalianMutationPromoterT7Available sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
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AAV-PB-SRT-tdTomato
Plasmid#154889PurposepiggyBac self-reporting transposon with tdTomato marker in AAV backboneDepositorInsertpiggyBac SRT
UseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-Ku70-siR-WT
Plasmid#82329PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertKu70 (XRCC6 Human)
UseTagsHAExpressionMammalianMutationPromoterCMV-tetAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-mDyn1 pcDNA3
Plasmid#36263DepositorInsertDynamin 1 (Dnm1 Mouse)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLgw-MBD3-V5-Dam
Plasmid#55605PurposeExpression of Dam fused MBD3 in mammalian cellsDepositorInsertMBD3 (MBD3 Human)
UseLentiviralTagsDamID (DNA adenine methyltransferase identificati…ExpressionMammalianMutationPromoterHSPAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
EF.CMV.RFP
Plasmid#17619DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCGN-MEF2D
Plasmid#32963PurposeFor mammalian expression of mouse MEF2D with an HA tagDepositorInsertMEF2D (Mef2d Mouse)
UseTagsHAExpressionMammalianMutationE287GPromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
GFP SMG1 WT
Plasmid#199571PurposeExpresses WT SMG1 fused to GFPDepositorInsertSMG1 WT (SMG1 Human)
UseTagsGFP fusion proteinExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
MLRV2: NF-kb-SMAD-DBE-P53-AP1
Plasmid#124536PurposeMultiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1DepositorInserts5 copies of the NF-kb DNA binding motif
7xSMAD_RE::FLuc
3xDBE_BE::Renilla (FOXO)
2xP53_RE::NLuc
6xAP-1_RE::GrRenilla
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-Ku70-siR-Mut6E
Plasmid#82330PurposePlasmid for constitutive or doxy-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.DepositorInsertKu70 (XRCC6 Human)
UseTagsHAExpressionMammalianMutationMut6E corresponding to K282E K287E T300E K331E K3…PromoterCMV-tetAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-BG4-NLS-mCer
Plasmid#211448Purposemammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequenceDepositorInsertAnti-DNA G-quadruplex single chain antibody BG4
UseTagsNLS-mCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only