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Plasmid#142186PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGRHL2 (GRHL2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Ezrin-N-14
Plasmid#55043PurposeLocalization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610DepositorInsertEzrin (EZR Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
ABE8.20-m
Plasmid#136300Purposeexpresses ABE8.20-m in mammalian cellsDepositorInsertABE8.20-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
V5-miniTurbo-NES_pCDNA3
Plasmid#107170Purposeexpresses V5-tagged miniTurbo in the mammalian cytosolDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionMammalianMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
ClopHensorN
Plasmid#50758PurposeMammalian expression vector expressing the ClopHensorN chloride and proton sensorDepositorInsertClopHensorN
UseTagsStrep-IIExpressionMammalianMutationE2GFP is EGFP-T203YPromoterCAGAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
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qTAG-C-dTAG-Puro
Plasmid#207727PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCherry-Alpha-5-Integrin-12
Plasmid#54970PurposeLocalization: Focal Adhesions, Excitation: 587, Emission: 610DepositorInsertAlpha-5 Integrin (ITGA5 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
mEGFP
Plasmid#18696DepositorInsertmonomeric EGFP
UseTagsExpressionMammalianMutationA206K mutationPromoterAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCE-hSK
Plasmid#41814PurposeNon-integrating (episomal) expression of human SOX2 and KLF4DepositorUseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-LKB1
Plasmid#8590DepositorInsertLKB1 (STK11 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pT2/SV-Venus-SIINFEKL
Plasmid#111624PurposeSleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studiesDepositorInsertVenus-LEQLE-SIINFEKL-TEW
UseTagsLEQLE-SIINFEKL-TEWExpressionMammalianMutationPromoterSV40Available sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
TPC2-G-GECO1.2
Plasmid#207141PurposeFusion of Ca2+ reporter and lysosomal Ca2+-permeable channelDepositorInsertTPC2N2 (TPCN2 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti MS2-P65-HSF1_Hygro
Plasmid#61426Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2DepositorInsertMS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLgw V5-EcoDam
Plasmid#59210PurposeMammalian DamID control lentiviral vector for expression of V5-Dam onlyDepositorInsertEcoDam
UseLentiviral; DamidTagsV5ExpressionMammalianMutationPromoterHeat Shock Minimal Promoter and heat shock minima…Available sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ZFN-AAVS1
Plasmid#89707PurposeMammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locusDepositorInsertZFN-AAVS1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgRNA-hSyn-mCherry
Plasmid#87916PurposesgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)DepositorInsertmCherry
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterhSynAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-GP
Plasmid#60495PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance geneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
Plasmid#222992PurposeMammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNADepositorInsertHuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
UseCRISPRTagsPuroExpressionMammalianMutationPromoterpCAGAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP hsp70
Plasmid#15215DepositorInserthsp70 (HSPA1A Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only