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Plasmid#39311DepositorInsertARID1A (ARID1A Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-YFP-raptor
Plasmid#73385PurposeExpresses YFP and HA tagged Human raptorDepositorInsertRegulatory Associated Protein Of MTOR, Complex 1 (RPTOR Human)
UseTagsHA and YFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVXT Sox4
Plasmid#101121PurposeSox4 coding regionDepositorInsertSOX4 (Sox4 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTag-RFP-C-h-Rab11a
Plasmid#79806PurposeExpress TagRFP labeled human Rab11a (GTPase located on recycling endosomal membranes).DepositorInsertRab11a (RAB11A Human)
UseTagsTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Ef1a-fDIO hChR2(H134R)-EYFP
Plasmid#55639PurposeFlp-Dependent ChR2-EYFPDepositorInserthChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHIV-H2BmRFP
Plasmid#18982DepositorInsertHistone H2B monomeric red fluorescent protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-100RPU.2-ETN-lox2272
Plasmid#154833PurposeDonor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of etanerceptDepositorInsertETN
UseCre/LoxTagsExpressionMammalianMutationPromoter100RPU.2Available sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUGW ECFP
Plasmid#128068PurposeLentiviral expression of ECFPDepositorInsertECFP
UseLentiviralTagsExpressionMammalianMutationnoPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
hfCas12Max
Plasmid#195336Purposevector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized hfCas12Max
UseTags3xFlagExpressionMammalianMutationN243R, E336R, D892RPromoterCBh, CMV, hU6Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG_hPPP1R15B_4-713_mCherry_UK1298
Plasmid#80707Purposemammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 4-713)DepositorInserthuman PPP1R15B (PPP1R15B Human)
UseTagsmCherryExpressionMammalianMutationCDS 4-713 no stop codonPromoterCMVAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti-mTagBFP2
Plasmid#216126PurposeLentiviral vector for expressing mTagBFP2.DepositorInsertEF1a-mTagBFP2-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF1436
Plasmid#143499PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFKBIZ (NFKBIZ Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLAI-Env
Plasmid#133996PurposeEnv gene from HIV-1 LAI in pcDNA3.1 expression vectorDepositorInsertEnvelope Glycoprotein
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)
Plasmid#185910PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpCas9-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9=D10APromoterCMV and T7Available sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLENTI EF1alpha FLAG/HA-PALI1
Plasmid#114445PurposeMammalian lentiviral expression vector containing N terminal FLAG/HA tagged human PALI1 coding sequenceDepositorInsertPALI1 (LCOR Human)
UseLentiviralTagsFLAG-HAExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-Nfil3
Plasmid#139815Purposedoxycycline-inducible expression of mouse Nfil3 in mammalian cellsDepositorInsertNfil3 (Nfil3 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-mCMVAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-P2A-EGFP (RTW4177)
Plasmid#139988PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337RPromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-BSD
Plasmid#174821PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-BSD markerDepositorInsertPEmax-P2A-BSD
UseTagsP2A-BSD, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF0295
Plasmid#142591PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMEF2A (MEF2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0672
Plasmid#141658PurposeLentiviral vector for overexpressing the ZNF827 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF827 (ZNF827 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only