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mCherry-Ezrin-N-14
Plasmid#55043PurposeLocalization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610DepositorInsertEzrin (EZR Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP
Plasmid#118156PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-EGFP
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine
Plasmid#228902PurposeFor AAV packaging and Cre-dependent expression of a peripherally restricted DREADDDepositorInserthydroxycarboxylic acid receptor 2 (Hcar2 Mouse)
UseAAV and Synthetic BiologyTagsHA and P2A mCitrineExpressionMammalianMutationR108KPromoterChicken Beta ActinAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-iLACCO1
Plasmid#218821PurposeMammalian expression of genetically encoded green fluorescent L-lactate biosensor with mild affinityDepositorInsertiLACCO1
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-BroccoliSamSensor#1
Plasmid#124363PurposeOverexpression of circular RNA (Broccoli-based SAM sensor)DepositorInsertTornado-BroccoliSamSensor#1
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-GFP-wt-lamin A
Plasmid#17662DepositorInsertGFP-lamin A (LMNA Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMLS-SV40-EGFP
Plasmid#46919PurposeTarget EGFP gene that is stably integrated into HEK293 genomeDepositorInsertGFP
UseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mtagBFP
Plasmid#89685PurposepCAG promoter driving mtagBFPDepositorInsertmtagBFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
iOn-CAG∞MCS
Plasmid#154013PurposeExpression vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019), equipped with an MCS to clone-in genes of interest and express it from a CAG promoterDepositorInsertMCS
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2
Plasmid#174665PurposeExpresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoterDepositorInsertsLUC2
mCherry:T2A:Hygro
UseLentiviral and LuciferaseTagsmCherry and hygromycin dual reporter gene linked …ExpressionMammalianMutationPromoterHuman cytomegalovirus immediate early enhancer/pr…Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE1
Plasmid#73019PurposeExpresses BE1 with C-terminal NLS in mammalian cellsDepositorInsertBE1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB
Plasmid#99391PurposeGateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)DepositorTypeEmpty backboneUseTagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTK96_mCherry-MRLC2
Plasmid#46358DepositorInsertMRLC2 (MYL12B Human)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterpBABEAvailable sinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
MGAT2-RpHLuorin2
Plasmid#171718PurposeCis-/medial-Golgi expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Galt1-Grx1-roGFP1-iE
Plasmid#230029PurposeExpressing Grx1-roGFP1-iE fluorescent fusion protein in mammalian cells with localization in the Golgi apparatus.DepositorInsertGalt1-Grx1-roGFP1-iE (B4GALT1 Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Pepper-fused sgRNA
Plasmid#217520PurposeExpresses Pepper-fused sgRNA in mammalian cells with U6 promoterDepositorInsertPepper-fused sgRNA for spCas9
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-jGCaMP8s-WPRE
Plasmid#179256PurposeAAV-mediated expression of GCaMP8s under the CAG promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE
Plasmid#121966PurposeExpresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.DepositorInsertscFv-GCN4
UseLentiviralTagssuperfold GFP-GB1-NLS-iLIDExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IZ-ANXA11-mRuby3
Plasmid#184894PurposeStable expression of ANXA11-mRuby3 (Annexin A11-mRuby3) in mammalian cellsDepositorInsertANXA11 (ANXA11 Human)
UseTagsmRuby3ExpressionMammalianMutationR230C relative to NP_001148.1PromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only
