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Showing: 2061 - 2080 of 71625 results

  1. pCag SE (Self-excising) FlpO-2A-Cre EV

    Plasmid
    #130986
    Purpose
    episomal expression of FlpO and Cre recombinases, self excising
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    FlpO-2A-Cre
    Use
    Cre/Lox and Unspecified; Episomal expression vect…
    Tags
    Expression
    Mammalian
    Mutation
    protamine intron added to FlpO to prevent bacteri…
    Promoter
    CMV/Chick β-actin (CAG)
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    130986-plasmid-map-sequence-id-304997

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  4. pSCAR_Cas9-blast_GFP

    Plasmid
    #162074
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-blasticidin S deaminase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162074-plasmid-map-sequence-id-325297

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  5. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

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  7. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE

    Plasmid
    #104491
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP7s
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104491-plasmid-map-sequence-id-200288

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  8. p-mCherry-C1-GtACR2

    Plasmid
    #85463
    Purpose
    Natural anion conducting Channelrhodopsin with blue absorption. Closing kinetics 60ms. High conductivity. Codon optimized for mammalian expression (human/mouse)
    Depositor
    Peter Hegemann
    Article
    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.
    Insert
    Synthetic construct ACR2 gene
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV (+enhancer)
    Available since
    Jan. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    85463-plasmid-map-sequence-id-170777

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  9. pAAV-CaMKII-STChRger2-TS-EYFP

    Plasmid
    #129393
    Purpose
    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    soma targeted ChRger2
    Use
    AAV
    Tags
    Kv2.1-TS-EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    129393-plasmid-map-sequence-id-258245

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  16. pNTI934

    Plasmid
    #216637
    Purpose
    Cal-ID-PM (V5) Plasma membrane-localized Cal-ID, mammalian expression and lentivirus production
    Depositor
    Nicholas Ingolia
    Article
    Kim et al Nat Chem Biol (2024)
    Insert
    Cal-ID-PM
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216637-plasmid-map-sequence-id-429983

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  17. pCS2+mScarlet3-C Cloning Vector

    Plasmid
    #220616
    Purpose
    To express a protein of interest fused to the C-terminus of mScarlet3 (unpublished)
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    mScarlet3 fusion
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    220616-plasmid-map-sequence-id-435943

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Showing: 2061 - 2080 of 71625 results