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Plasmid#163930PurposeExpression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt13C(S473D)
Plasmid#86577PurposeExpresses cleavable human full-length Akt1(S473D) phosphomimeticDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130; S473D phos…PromoterAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVMG0146_Cq-U6-1_2xBbsI-gRNA
Plasmid#169238PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertCq-U6-1_2xBbsI-gRNA
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ039653Available sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0149_Cq-U6-6_2xBbsI-gRNA
Plasmid#169323PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0149_Cq-U6-6_2xBbsI-gRNA
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ039596Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACUH-sfcherry2(1-10)
Plasmid#172061PurposeUAS vector to express sfcherry2(1-10) in DrosophilaDepositorInsertsfcherry2(1-10)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDSAP
Plasmid#62293Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, Puromycin resistance markerDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
BacPAK Flag-NFRKB
Plasmid#19791DepositorInsertNFRKB (NFRKB Human)
UseTagsFlag and HisExpressionInsectMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
GFP-BICD2N
Plasmid#64070PurposeN-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)DepositorInsertBICD2N (1-400) (Bicd2 Mouse)
UseTagsGFP and His-ZZ-TCSExpressionInsectMutationCodon optimized for Sf9 expressionPromoterAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC158-3XUAS-IVS-B3::PEST
Plasmid#32138DepositorInsertB3::PEST
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His-Tipin
Plasmid#22891DepositorInsertTipin (TIPIN Human)
UseTagsHisExpressionInsectMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLOT-syb-sp-YFP1-10
Plasmid#73700PurposeNeuronal-synaptobrevin (syb):spYFP1–10 chimera for synaptic GRASPDepositorInsertNeuronal-synaptobrevin:spYFP1–10 chimera
UseLexaop reporter vectorTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2Bac.V5.p62
Plasmid#39218DepositorInsertp62 (GTF2H1 Human)
UseTagsV5ExpressionInsectMutationPromoterPp10Available sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJC-mhc2.4kb-TALE3-VP64-P10
Plasmid#104611PurposeExpresses TALE3 under the control of a 2.4kb mhc enhancer elementDepositorInsert2.4kb mhc enhancer element (Mhc Fly)
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUWR-Cad-Venus
Plasmid#58328PurposeDestination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genomeDepositorInsertCad-Venus
UseTagsExpressionInsectMutationPromoterpoly-ubiquitinAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_His-ZZ-TEV-Lis1-SNAPf
Plasmid#133073PurposePlasmid for insect cell expression of human full-length LIS1 with an N terminal His-ZZ-LTLT tag and C terminal SNAPf tagDepositorInsertLis1 (PAFAH1B1 Human)
UseTags8x His, Linker-TEV-linker_TEV, SNAPf, and ZZExpressionInsectMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC93
Plasmid#118911Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6DepositorInsertNcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
UseTagseGFP-HIS6ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-VAV2 DH/PH/C1
Plasmid#81060Purposemammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domainDepositorInsertsUseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR R4 ATCC LacZ GCTT
Plasmid#173668PurposeDestination vector for Golden Gate Cloning assemblies, blue-white screeningDepositorTypeEmpty backboneUseTagsExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
ULK3 (ULK3A-c002)
Plasmid#110272PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertULK3 (ULK3 Human)
UseTagsHis6-ZBasic-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINTF
Plasmid#41114DepositorTypeEmpty backboneUseTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only