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Plasmid#78899PurposeExpresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell cultureDepositorInsertdCas9-10XGCN4
UseCRISPRTagsHAExpressionInsectMutationPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40]
Plasmid#86453PurposeUAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Transformation plasmid; Red eye marker)DepositorInsert3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-GL-DSCP
Plasmid#83338Purposefor Gateway cloning of enhancer elements upstream of the DSCP and GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsGFP::Luciferase(nls)ExpressionInsectMutationPromoterDrosophila Synthetic Core PromoterAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR4 mito roGFP2-Grx1
Plasmid#65000PurposeExpression of mitochondrial roGFP2-Grx1 under the alphaTub84B promoterDepositorInsertGlutaredoxin-1 (GLRX Human)
UseTagsroGFP2ExpressionInsectMutationmitochondrial targeting sequencePromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNos-PE2-attB
Plasmid#149549PurposeExpression of PE2 enzyme under control of the germ cell specific nanos promoter. Can be used to generate transgenic flies with vermillion+ selection.DepositorInsertPE2
UseCRISPRTagsExpressionInsectMutationPromoternanos (nos)Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAC-ECFP-15xQUAS_TATA-SV40
Plasmid#104875PurposepiggyBac vector for cloning 15xQUAS transgenes; used for mosquito tranformationDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC6-15XUAS-IVS-mCD8::GFP
Plasmid#26219DepositorInsertmCD8::GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr-D435A/E437A
Plasmid#40829PurposeExpresses Flag, Myc tagged mutant Nbr in Drosophila cellsDepositorInsertNbr D435A/E437A (Nbr Fly)
UseTags3xFLAG and 6xMycExpressionInsectMutationD435A/E437A, part of the DEDD motif; mutations p…PromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mPA-GFP-PA-Rac1
Plasmid#22022PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
UseTags6X His and mPA-GFPExpressionBacterial, Insect, and Mamm…MutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUAS-Myc-BirA-G3-ER
Plasmid#173815PurposePromiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage siteDepositorInsertMyc-BirA-G3-ER
UseTagsMycExpressionInsectMutationPromoterUASAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2Bac.HA.p52
Plasmid#39219DepositorInsertp52 (GTF2H4 Human)
UseTagsHAExpressionInsectMutationPromoterPp10Available sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
1154B_gFLE_pB_VTK_ActG
Plasmid#187238PurposeAn. gambiae transgenesis plasmid. PiggyBac with transposase in backbone. Expresses two gRNAs targeting fleDepositorInsertFemaleless
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBac-DsRed-ORCO_9kbProm-QF2
Plasmid#104877PurposeAnopheles gambiae ORCO enhancer/promter to drive QF2 expression in Anopheles transgenic mosquitoesDepositorInsertAgORCO-QF2
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAct-GW-HygroR
Plasmid#149610PurposeGateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.DepositorInsertGateway cassette (ccdb + Chlor.R.)
UseTagsExpressionInsectMutationPromoterAct5cAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pUbi-p63e eGFP SV40
Plasmid#132650PurposeExpresses eGFP mRNA from the Drosophila Ubi-p63e promoterDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterUbi-p63eAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCoofy63
Plasmid#122015PurposeBaculovirus vector for parallel SLIC cloning containing N-terminal His6-Smt3Star (SumoStar*) and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis6-Smt3Star and Multi3: depending on the LP2 pr…ExpressionInsectMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
dVGlut-Gal80
Plasmid#52555PurposeExpresses Gal80 under vesicular glutamate transporter promoterDepositorUseTagsExpressionInsectMutationPromoterDrosophila Synthetic Core PromoterAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac SpyTag-β2AR-SpyTag
Plasmid#135620PurposeExpression of SpyTag-B2AR in insect cellDepositorInsertBeta 2 adrenergic receptor (ADRB2 Human)
UseTagsHA-FLAG tag, Spytag, and eGFP-10xHis-R1D4 tagExpressionInsectMutationDeleted 1-24 aa, 366-413 aaPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Brp-T2A-QF2w-Hsp70-3XP3-dsRed
Plasmid#141094PurposeTemplate plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.DepositorInsertbruchpilot
UseCRISPRTagsExpressionInsectMutationcoding sequence downstream of CRISPR cut site, sy…PromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT Draper-mCherry
Plasmid#126667PurposeInducible expression of Draper-mCherry in S2 cellsDepositorInsertDraper mCherry
UseTagsExpressionInsectMutationC813Y- see depositor comments belowPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only