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Plasmid#160274PurposeC-term 3C cleavable GST tag for insect cell expressionDepositorTypeEmpty backboneUseTagsGSTExpressionInsectMutationPromoterpolyhydrinAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only
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908I
Plasmid#160241PurposeVanO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-SV40-Ubiquitin-Renilla-attBDepositorInsertsVan Operator (2x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter
UseTagsT2A GFPExpressionInsectMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
907F1
Plasmid#160233PurposeFlightN-VanR-VP16-3X-SV40-attBDepositorInsertsVanR
Flightin promoter
UseTagsVP16ExpressionInsectMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
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OA-986C
Plasmid#125000PurposeExpresses dCas9-VPR driven by the bottleneck promoterDepositorInsertdCas9-VPR
UsePiggybackTagsExpressionInsectMutationPromoterbottleneckAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-986D
Plasmid#125001PurposeExpresses dCas9-VP64 driven by the Ubiquitin-63E promoterDepositorInsertdCas9-VP64
UsePiggybackTagsExpressionInsectMutationPromoterubiquitinAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-986E
Plasmid#125002PurposeExpresses dCas9-VP64 driven by the bottleneck promoterDepositorInsertdCas9-VP64
UsePiggybackTagsExpressionInsectMutationPromoterbottleneckAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pUC57-attB2-SA(2)-CRY2-mCherry
Plasmid#160441PurposeTo create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 2.DepositorInsertCryptochrome 2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionInsectMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-attB2-SA(1)-CRY2-mCherry
Plasmid#160440PurposeTo create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 1.DepositorInsertCryptochrome 2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionInsectMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-attB2-SA(0)-CRY2-mCherry
Plasmid#160439PurposeTo create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 0.DepositorInsertCryptochrome 2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionInsectMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
R979-X57-008: Hs.KRAS4b(1-169)
Plasmid#159690PurposeBaculovirus protein expression of KRAS4b (1-169)DepositorInsertHs.KRAS4b(1-169) (KRAS Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X30-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D
Plasmid#159557PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G13DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G13D (KRAS Human)
UseTagsHis6-MBP-tevExpressionInsectMutationG13DPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X32-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R
Plasmid#159559PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61RDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R (KRAS Human)
UseTagsHis6-MBP-tevExpressionInsectMutationQ61RPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X28-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D
Plasmid#159555PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)
UseTagsHis6-MBP-tevExpressionInsectMutationG12DPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X31-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L
Plasmid#159558PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61LDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L (KRAS Human)
UseTagsHis6-MBP-tevExpressionInsectMutationQ61LPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-URH49 MUT
Plasmid#110126PurposeExpresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activityDepositorInsertURH49 (DDX39A Human)
UseTagsFlagExpressionInsectMutation4 amino acid mutations (RSFS motif changed to KSL…PromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E MUT
Plasmid#110122PurposeExpresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activityDepositorInsertHel25E (Hel25E Fly)
UseTagsFlagExpressionInsectMutation4 mutations (KKLN motif changed to RSFS)PromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only