OA-1045E
(Plasmid
#125007)
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PurposeExpresses tRNA-flanked gRNAs targeting hid, eve, and hedgehog
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 125007 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneattB
- Backbone size w/o insert (bp) 7473
- Total vector size (bp) 8905
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeve gRNA
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gRNA/shRNA sequenceATCGTGCGGTGCTGAGAG
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SpeciesD. melanogaster (fly)
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Entrez Geneeve (a.k.a. Dmel_CG2328, 10.5, 10.9, 14.10, 20.35, CG2328, Dm-eve, Dmel\CG2328, E(eve), EVE, Eve, Even, F, V, VI, dm-eve, eve2, even, l(2)46CFg, l(2)46CFh, l(2)46CFj, l(2)46CFp, l(2)46Ce, l(2)46Cg)
- Promoter U6:3
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer aaaaatggtgggcataatagtgttgtt
- 3′ sequencing primer ggCTTCGAGACCGTGACCTACATC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OA-1045E was a gift from Omar Akbari (Addgene plasmid # 125007 ; http://n2t.net/addgene:125007 ; RRID:Addgene_125007) -
For your References section:
Engineered reproductively isolated species drive reversible population replacement. Buchman A, Shriner I, Yang T, Liu J, Antoshechkin I, Marshall JM, Perry MW, Akbari OS. Nat Commun. 2021 Jun 2;12(1):3281. doi: 10.1038/s41467-021-23531-z. 10.1038/s41467-021-23531-z PubMed 34078888