-
Plasmid#147007PurposeInsect Expression of DmUPF3-K31E-siRNAresDepositorInsertDmUPF3-K31E-siRNAres (Upf3 Fly)
UseTagsExpressionInsectMutation5 silent and one non silent mutation P305S compar…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF1_92-1180-siRNAres-V5His6_N
Plasmid#146990PurposeInsect Expression of DmUPF1_92-1180-siRNAresDepositorInsertDmUPF1_92-1180-siRNAres (Upf1 Fly)
UseTagsExpressionInsectMutation1 non silent mutations: G995E compared to the seq…PromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-K690A_M
Plasmid#146957PurposeInsect Expression of DmAGO1-K690ADepositorInsertDmAGO1-K690A (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-nerfin-HSL_E
Plasmid#146228PurposeInsect Expression of Nerfin-3UTRDepositorInsertNerfin-3UTR (nerfin-1 Fly)
UseTagsExpressionInsectMutationNucleotide mutations A>T 4387 and A>T 4955 …PromoterAvailable sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCup_1-417-Y342AL347A-V5His6_M
Plasmid#146901PurposeInsect Expression of DmCup_1-417-Y342AL347ADepositorInsertDmCup_1-417-Y342AL347A (cup Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMe31B-mut1_E
Plasmid#146203PurposeInsect Expression of DmMe31B-mut1DepositorInsertDmMe31B-mut1 (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+Q111R
Plasmid#167173PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R mutation in Sf9 cellsDepositorInsertsATP1A1-S+Q111R
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged Q at 111 to RPromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+N122D
Plasmid#167174PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with N122D mutation in Sf9 cellsDepositorInsertsATP1A1-S+N122D
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged N at 111 to DPromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+Q111R+N122D
Plasmid#167175PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R and N122D mutations in Sf9 cellsDepositorInsertsATP1A1-S+Q111R+N122D
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged Q at 111 to R and changed N at 122 to DPromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+10subs
Plasmid#167176PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 10 R mutations in Sf9 cellsDepositorInsertsATP1A1-S+10R
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged A at 112 to T; E at 116 to D; I at 135 to…PromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+12subs
Plasmid#167177PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 12 R mutations in Sf9 cellsDepositorInsertsATP1A1-S+12R
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged Q at 111 to R; A at 112 to T; E at 116to …PromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-gamma-tubulin(N229A)-TEV-His6
Plasmid#178077PurposeInsect cell expression of gamma-tubulin(N229A)-TEV-His6DepositorInsertgamma-tubulin with an N229A point mutation (TUBG1 Human)
UseTagsTEV-His6ExpressionInsectMutationChanged Asparagine 229 to AlaninePromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-ZZ-TEV-MZT2-3C-mEGFP
Plasmid#178074PurposeInsect cell expression of ZZ-TEV-MZT2-3C-mEGFPDepositorInsertMZT2A (MZT2A Human)
UseTagsPreScission-mEGFP and ZZ-TEVExpressionInsectMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-GCP4
Plasmid#178070PurposeInsect cell expression of GCP4DepositorInsertGCP4 (TUBGCP4 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-gamma-tubulin-TEV-His6
Plasmid#178067PurposeInsect cell expression of gamma-tubulin-TEV-His6DepositorInsertgamma-tubulin (TUBG1 Human)
UseTagsTEV-His6ExpressionInsectMutationPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACE-Truncated-Kir3.2-StreptagII
Plasmid#177263PurposeExpresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cellsDepositorInsertG-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)
UseTagsStreptagIIExpressionInsectMutationResidues 49-378Promoterpolyhedrin promoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
RABGEF1A-c014
Plasmid#175478PurposeProtein expression in insect cells. Entire CDS, M1-G491. Codon optimised.DepositorInsertRABGEF1 (RABGEF1 Human)
UseTagsHis6-Zbasic-TEVExpressionInsectMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
RABEP1A-c019
Plasmid#175477PurposeProtein expression in insect cells. Entire CDS, M1-T862. Codon optimised.DepositorInsertRABEP1 (RABEP1 Human)
UseTagsTEV-TwinStrepExpressionInsectMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOmnibac_ZZ_linker_K490_GFP_SNAP
Plasmid#159721PurposeExpresses kinesin-1 (amino acids 1-490) in Sf9 cells with a C-terminal GFP and SNAP tagDepositorInsertKIF5B (only amino acids 1-490), K490 (KIF5B Human)
UseTagsGFP and SNAP tags and ZZ affinity tagExpressionInsectMutationOnly amino acids (1-490)PromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only