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Plasmid#218233PurposeHDR plasmid for inserting Ceratitis capitata transformer female intron into the white pupae geneDepositorInsertputative metabolite transport protein
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10
Plasmid#218864PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8s
UseTags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10
Plasmid#218866PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8f
UseTags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationQ315LPromoterhsp70Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-BacMam-4x-PyOtR
Plasmid#217364PurposeExpresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmidDepositorInsert4 copies of pyrrolysyl-tRNA mutant PyOtR
UseBaculoviralTagsExpressionInsect and MammalianMutationPromoterU6Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-GFP(39TAG)
Plasmid#217360PurposeExpression of reporter EGFP with a TAG stop codon at 39 aa position; can be packaged into bacmidDepositorInsertEnhanced green fluorescent protein
UseBaculoviralTagsExpressionInsect and MammalianMutationY39TAGPromoterCMVAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOET5 rota-VLP
Plasmid#218147PurposeForms rotavirus-like particles in insect cells by expression of VP6 (GenBank ID: ACL93331.1).DepositorInsertRotavirus VP6
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOET5 CVB3-VLP
Plasmid#218146PurposeForms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).DepositorInsertsVP1–VP4
3CD
UseTagsExpressionInsectMutationPromoterCMV and PolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOET5 GII.4 Sydney noro-VLP
Plasmid#218092PurposeForms Sydney GII.4 Sydney norovirus-like particle in insect cells by expression of VP1 from strain GII.4/Sydney/NSW0514/2012/AU (GenBank ID: AFV08795).DepositorInsertnoro-VLP
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-3C-hTspan12LEL(118-218)_pAcGP67a
Plasmid#216383PurposeBaculovirus transfer vector to secrete human Tspan12 LEL (residues 118-218) fused to MBPDepositorInsertTetraspanin-12 (TSPAN12 Human)
UseBaculovirusTagsGP64 signal sequence-MBP-3CExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
mFzd4CRDL(42-203)-6xHis_pAcGP67a
Plasmid#216379PurposeBaculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cellsDepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTags6xHis and GP64 signal sequenceExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
I14-pAc5.1 lambda N HA Cup
Plasmid#206493PurposeCup expression forSchneider cell imagingDepositorTypeEmpty backboneUseTagslambda N HAExpressionInsectMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
F23-pAc5.1 EGFP Cup
Plasmid#206488PurposeCup expression for Schneider cell imagingDepositorInsertCup
UseTagsEGFPExpressionInsectMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
F17-pAc5.1 EGFP Vasa 1-200
Plasmid#206484Purposevasa 1-200 expression for Schneider cell imagingDepositorInsertVasa 1-200
UseTagsEGFPExpressionInsectMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDB_052
Plasmid#216082Purposefor stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous insertion, destination vectorDepositorInsertHygromycin resistance gene
UseDestinationTagsExpressionInsectMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDB_051
Plasmid#216081Purposefor stable fly cell lines; attL and attR sequences for genome integration by phiC31, destination vectorDepositorInsertattL and attR sequences for genome integration by phiC31
UseDestinationTagsExpressionInsectMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDB_053
Plasmid#216083Purposefor transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vectorDepositorInsertgypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
UseDestinationTagsExpressionInsectMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual-GST-TEV-SINTBAD-mCherry
Plasmid#208874PurposeFor expression in insect cells of SINTBAD-mCherryDepositorInsertSINTBAD (TBKBP1 Human)
UseTagsGST-TEVExpressionInsectMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual- GST-TEV-TBK1
Plasmid#208875PurposeFor expression in insect cells of TBK1DepositorInsertTBK1 (TBK1 Human)
UseTagsGST-TEVExpressionInsectMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1
Plasmid#216378PurposeBaculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)DepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTagsHA signal sequence-FLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-LIC_MAGEA3:104-314
Plasmid#210941PurposeInsect expression of human MAGEA3 fragment E104 to E314. Untagged.DepositorInsertMAGEA3:E104-E314 (MAGEA3 Human)
UseTagsExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only